Molecular Cloning: A Laboratory Manual, Volume 1 |
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Results 1-3 of 7
Page 1-15
... Bgll Eco0109 2287 2223 Aatil 2105 Sspl Sphl 2567 2000 PSP64 / PSP65 ( 3 kb ) 1000 HgiEll 988 SP6 promoter Polycloning site Pvull 232 Io ori Afflll 407 1 2 3 4 5 6 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 7 8 Thr Asn Ser Ser Ser Pro Thr ...
... Bgll Eco0109 2287 2223 Aatil 2105 Sspl Sphl 2567 2000 PSP64 / PSP65 ( 3 kb ) 1000 HgiEll 988 SP6 promoter Polycloning site Pvull 232 Io ori Afflll 407 1 2 3 4 5 6 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 7 8 Thr Asn Ser Ser Ser Pro Thr ...
Page 1-17
... Bgll pGEM - 3Z ( 2.74 kb ) Polycloning Sites pGEM - 3Z T7 transcription start ori 1000 Plac Bgll 2576 Mstl 2583 Pvul 2604 Pvull 2633 lacz Pvull 269 SP6 transcription start ...
... Bgll pGEM - 3Z ( 2.74 kb ) Polycloning Sites pGEM - 3Z T7 transcription start ori 1000 Plac Bgll 2576 Mstl 2583 Pvul 2604 Pvull 2633 lacz Pvull 269 SP6 transcription start ...
Page 1-18
... kb ) 1560 Mstl 1458 Bgll ori Sspl 2978 IG Sspl 3002 Bg / l 3032 Mstl 3039 Pvul 3060 Pvull 3089 ད lacz T7 promoter Polycloning site SP6 promoter Plac Pvull 269 Polycloning Site T7 transcription start 1000 P SP6 transcription start ...
... kb ) 1560 Mstl 1458 Bgll ori Sspl 2978 IG Sspl 3002 Bg / l 3032 Mstl 3039 Pvul 3060 Pvull 3089 ད lacz T7 promoter Polycloning site SP6 promoter Plac Pvull 269 Polycloning Site T7 transcription start 1000 P SP6 transcription start ...
Contents
BOOK | 1-2 |
DEVELOPMENT OF PLASMID CLONING VECTORS | 1-7 |
Index | 1-8 |
Copyright | |
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agar agarose gel aliquots amber mutations bacteria bacteriophage M13 bacteriophage particles bacteriophage T4 bacteriophage T4 DNA BamHI Bgill buffer cDNA cells centrifugation at 12,000g cleaved cloning coli DNA polymerase colonies containing cosmid cosmid vector culture digestion DNA fragments DNA ligase DNA molecules DNA polymerase double-stranded DNA EcoRI EDTA efficiency Escherichia coli ethanol ethidium bromide eukaryotic eukaryotic DNA exonuclease extracts foreign DNA gel electrophoresis gene genetic Gp Cp HindIII Hindill host hybridization Incubate infected inserted intergenic region Kpnl lacZ linear lysogenic method methylase methylation microfuge tube minutes at 4°C nin5 nitrocellulose nucleotides origin of replication packaging pellet phagemids plaques plasmid plasmid DNA plate polycloning prepared protein Pvul recA recombinant red gam remove replication restriction enzyme room temperature Sacl Sall single-stranded DNA Smal solution sterile stored strains strand supernatant T4 DNA ligase teriophage Transfer Tris Cl pH vector DNA vitro volume Xbal µg/ml