Molecular Cloning: A Laboratory Manual, Volume 1 |
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Page vi
... Chapter 7 ; Brian Seed for permission to include a description of his unpublished procedure for screening libraries by recombination ( Chapter 10 ) and many other useful suggestions ; Doug Hanahan for advice on trans- formation ( Chapter ...
... Chapter 7 ; Brian Seed for permission to include a description of his unpublished procedure for screening libraries by recombination ( Chapter 10 ) and many other useful suggestions ; Doug Hanahan for advice on trans- formation ( Chapter ...
Page viii
... chapters on DNA sequencing and expression of cloned genes in mam- malian cells , respectively ; and to Judy Campbell , who contributed signifi- cantly to the preparation of the chapter on enzymes . We are also grateful to Mike Ockler ...
... chapters on DNA sequencing and expression of cloned genes in mam- malian cells , respectively ; and to Judy Campbell , who contributed signifi- cantly to the preparation of the chapter on enzymes . We are also grateful to Mike Ockler ...
Page viii
... chapters on DNA sequencing and expression of cloned genes in mammalian cells , respectively ; and to Judy Campbell , who contributed significantly to the preparation of the chapter on enzymes . We are also grateful to Mike Ockler , who ...
... chapters on DNA sequencing and expression of cloned genes in mammalian cells , respectively ; and to Judy Campbell , who contributed significantly to the preparation of the chapter on enzymes . We are also grateful to Mike Ockler , who ...
Contents
BOOK | 1-2 |
DEVELOPMENT OF PLASMID CLONING VECTORS | 1-7 |
Index | 1-8 |
Copyright | |
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Common terms and phrases
agar agarose gel aliquots amber mutations bacteria bacteriophage M13 bacteriophage particles bacteriophage T4 bacteriophage T4 DNA BamHI Bgill buffer cDNA cells centrifugation at 12,000g cleaved cloning coli DNA polymerase colonies containing cosmid cosmid vector culture digestion DNA fragments DNA ligase DNA molecules DNA polymerase double-stranded DNA EcoRI EDTA efficiency Escherichia coli ethanol ethidium bromide eukaryotic eukaryotic DNA exonuclease extracts foreign DNA gel electrophoresis gene genetic Gp Cp HindIII Hindill host hybridization Incubate infected inserted intergenic region Kpnl lacZ linear lysogenic method methylase methylation microfuge tube minutes at 4°C nin5 nitrocellulose nucleotides origin of replication packaging pellet phagemids plaques plasmid plasmid DNA plate polycloning prepared protein Pvul recA recombinant red gam remove replication restriction enzyme room temperature Sacl Sall single-stranded DNA Smal solution sterile stored strains strand supernatant T4 DNA ligase teriophage Transfer Tris Cl pH vector DNA vitro volume Xbal µg/ml