Molecular Cloning: A Laboratory Manual, Volume 1 |
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Page 5-40
... fragments that are to be used as substrates for ligation . The 5 ' → 3 ′ exonuclease activity can be removed proteolytically from the holoenzyme without affecting either the polymerase ... Fragment of DNA Polymerase I (Klenow Fragment) 5 40.
... fragments that are to be used as substrates for ligation . The 5 ' → 3 ′ exonuclease activity can be removed proteolytically from the holoenzyme without affecting either the polymerase ... Fragment of DNA Polymerase I (Klenow Fragment) 5 40.
Page 6-22
... DNA FRACTIONATED ON AGAROSE GELS Although many methods have been developed over the years to recover DNA from gels ... fragments of DNA . The efficiency with which DNA is recovered from agarose gels is a function of its molecular weight ...
... DNA FRACTIONATED ON AGAROSE GELS Although many methods have been developed over the years to recover DNA from gels ... fragments of DNA . The efficiency with which DNA is recovered from agarose gels is a function of its molecular weight ...
Page 6-60
... DNA fragments produced by cleavage of A DNA with endonuclease RI . Nature 241 : 120 . Anand , R. 1986. Pulsed field gel elec- trophoresis : A technique for fractionat- ing large DNA molecules . Trends Genet . 2 : 278 . Bensaude , O ...
... DNA fragments produced by cleavage of A DNA with endonuclease RI . Nature 241 : 120 . Anand , R. 1986. Pulsed field gel elec- trophoresis : A technique for fractionat- ing large DNA molecules . Trends Genet . 2 : 278 . Bensaude , O ...
Contents
BOOK | 1-2 |
DEVELOPMENT OF PLASMID CLONING VECTORS | 1-7 |
Index | 1-8 |
Copyright | |
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Common terms and phrases
agar agarose gel aliquots amber mutations bacteria bacteriophage M13 bacteriophage particles bacteriophage T4 bacteriophage T4 DNA BamHI Bgill buffer cDNA cells centrifugation at 12,000g cleaved cloning coli DNA polymerase colonies containing cosmid cosmid vector culture digestion DNA fragments DNA ligase DNA molecules DNA polymerase double-stranded DNA EcoRI EDTA efficiency Escherichia coli ethanol ethidium bromide eukaryotic eukaryotic DNA exonuclease extracts foreign DNA gel electrophoresis gene genetic Gp Cp HindIII Hindill host hybridization Incubate infected inserted intergenic region Kpnl lacZ linear lysogenic method methylase methylation microfuge tube minutes at 4°C nin5 nitrocellulose nucleotides origin of replication packaging pellet phagemids plaques plasmid plasmid DNA plate polycloning prepared protein Pvul recA recombinant red gam remove replication restriction enzyme room temperature Sacl Sall single-stranded DNA Smal solution sterile stored strains strand supernatant T4 DNA ligase teriophage Transfer Tris Cl pH vector DNA vitro volume Xbal µg/ml