Molecular Cloning: A Laboratory Manual, Volume 1 |
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Results 1-3 of 52
Page 1-54
... HindIII . The resulting circular recombinant is then used to transform E. coli to ampicillin resistance . Because of the lack of com- plementarity between the HindIII and BamHI protruding ends , the vector fragment cannot circularize ...
... HindIII . The resulting circular recombinant is then used to transform E. coli to ampicillin resistance . Because of the lack of com- plementarity between the HindIII and BamHI protruding ends , the vector fragment cannot circularize ...
Page 2-23
... HindIII sites . Charon 32 can also be used as a HindIII substitution vector or as an insertion vector to clone fragments up to 9 kb in length in the unique SacI site . The vector and recombinants are able to grow in recA hosts because ...
... HindIII sites . Charon 32 can also be used as a HindIII substitution vector or as an insertion vector to clone fragments up to 9 kb in length in the unique SacI site . The vector and recombinants are able to grow in recA hosts because ...
Page 2-35
... HindIII , and EcoRI ) and is used to clone large ( 10 kb to 22 kb ) DNA fragments . The polycloning - site sequences are inverted with respect to each other . Inserts that destroy the XhoI or BamHI site can be excised at flanking sites ...
... HindIII , and EcoRI ) and is used to clone large ( 10 kb to 22 kb ) DNA fragments . The polycloning - site sequences are inverted with respect to each other . Inserts that destroy the XhoI or BamHI site can be excised at flanking sites ...
Contents
BOOK | 1-2 |
DEVELOPMENT OF PLASMID CLONING VECTORS | 1-7 |
Index | 1-8 |
Copyright | |
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Common terms and phrases
agar agarose gel aliquots amber mutations bacteria bacteriophage M13 bacteriophage particles bacteriophage T4 bacteriophage T4 DNA BamHI Bgill buffer cDNA cells centrifugation at 12,000g cleaved cloning coli DNA polymerase colonies containing cosmid cosmid vector culture digestion DNA fragments DNA ligase DNA molecules DNA polymerase double-stranded DNA EcoRI EDTA efficiency Escherichia coli ethanol ethidium bromide eukaryotic eukaryotic DNA exonuclease extracts foreign DNA gel electrophoresis gene genetic Gp Cp HindIII Hindill host hybridization Incubate infected inserted intergenic region Kpnl lacZ linear lysogenic method methylase methylation microfuge tube minutes at 4°C nin5 nitrocellulose nucleotides origin of replication packaging pellet phagemids plaques plasmid plasmid DNA plate polycloning prepared protein Pvul recA recombinant red gam remove replication restriction enzyme room temperature Sacl Sall single-stranded DNA Smal solution sterile stored strains strand supernatant T4 DNA ligase teriophage Transfer Tris Cl pH vector DNA vitro volume Xbal µg/ml