Molecular Cloning: A Laboratory Manual, Volume 1 |
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Page 5-92
... Nucleic Acids Res . 15 : 3199 . Kornberg , T. and A. Kornberg . 1974 . Bacterial DNA polymerases . In The en- zymes , 3rd edition ( ed . P.D. Boyer ) , vol . 10 , p . 119. Academic Press , New York . Kotewicz , M.L. , C.M. Sampson ...
... Nucleic Acids Res . 15 : 3199 . Kornberg , T. and A. Kornberg . 1974 . Bacterial DNA polymerases . In The en- zymes , 3rd edition ( ed . P.D. Boyer ) , vol . 10 , p . 119. Academic Press , New York . Kotewicz , M.L. , C.M. Sampson ...
Page 5-94
... Nucleic Acids Res . 16 : 1563 . Richardson , C.C. 1971. Polynucleotide ki- nase from Escherichia coli infected with bacteriophage T4 . In Procedures in nucleic acid research ( ed . G.L. Can- toni and D.R. Davies ) , vol . 2 , p . 815 ...
... Nucleic Acids Res . 16 : 1563 . Richardson , C.C. 1971. Polynucleotide ki- nase from Escherichia coli infected with bacteriophage T4 . In Procedures in nucleic acid research ( ed . G.L. Can- toni and D.R. Davies ) , vol . 2 , p . 815 ...
Page 5-94
... nucleic acid research ( ed . G.L. Can- Setlow , P. 1976. Nearest neighbor fretoni and D.R. Davies ) , vol . 2 , p . 815 . quencies in deoxyribonucleic acids . In Harper and Row , New York . Handbook of biochemistry and molecu1981 ...
... nucleic acid research ( ed . G.L. Can- Setlow , P. 1976. Nearest neighbor fretoni and D.R. Davies ) , vol . 2 , p . 815 . quencies in deoxyribonucleic acids . In Harper and Row , New York . Handbook of biochemistry and molecu1981 ...
Contents
BOOK | 1-2 |
DEVELOPMENT OF PLASMID CLONING VECTORS | 1-7 |
Index | 1-8 |
Copyright | |
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Common terms and phrases
agar agarose gel aliquots amber mutations bacteria bacteriophage M13 bacteriophage particles bacteriophage T4 bacteriophage T4 DNA BamHI Bgill buffer cDNA cells centrifugation at 12,000g cleaved cloning coli DNA polymerase colonies containing cosmid cosmid vector culture digestion DNA fragments DNA ligase DNA molecules DNA polymerase double-stranded DNA EcoRI EDTA efficiency Escherichia coli ethanol ethidium bromide eukaryotic eukaryotic DNA exonuclease extracts foreign DNA gel electrophoresis gene genetic Gp Cp HindIII Hindill host hybridization Incubate infected inserted intergenic region Kpnl lacZ linear lysogenic method methylase methylation microfuge tube minutes at 4°C nin5 nitrocellulose nucleotides origin of replication packaging pellet phagemids plaques plasmid plasmid DNA plate polycloning prepared protein Pvul recA recombinant red gam remove replication restriction enzyme room temperature Sacl Sall single-stranded DNA Smal solution sterile stored strains strand supernatant T4 DNA ligase teriophage Transfer Tris Cl pH vector DNA vitro volume Xbal µg/ml