Molecular Cloning: A Laboratory Manual, Volume 1 |
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Page 2-23
... SacI site . The vector and recombinants are able to grow in recA hosts because they carry gam . There is no selection for recombinants . It is also possible to use this vector as a substitution vector for EcoRI - SalI , SalI - XhoI ...
... SacI site . The vector and recombinants are able to grow in recA hosts because they carry gam . There is no selection for recombinants . It is also possible to use this vector as a substitution vector for EcoRI - SalI , SalI - XhoI ...
Page 2-37
... SacI , and XhoI ) and is used to clone large ( 9 kb to 22 kb ) DNA fragments . The polycloning - site sequences are ... SacI , bacteriophage T7 promoter , Sall , XhoI , EcoRI , XbaI - stuffer - XbaI , EcoRI , XhoI , SalI , bacteriophage ...
... SacI , and XhoI ) and is used to clone large ( 9 kb to 22 kb ) DNA fragments . The polycloning - site sequences are ... SacI , bacteriophage T7 promoter , Sall , XhoI , EcoRI , XbaI - stuffer - XbaI , EcoRI , XhoI , SalI , bacteriophage ...
Page 2-49
... SacI ' , Sall , and EcoRI . The two vectors differ only in the orientation of the polycloning site . They have been ... SacI site in lacZ . If you plan to use the SacI site , make sure you have the version from which the extra SacI site ...
... SacI ' , Sall , and EcoRI . The two vectors differ only in the orientation of the polycloning site . They have been ... SacI site in lacZ . If you plan to use the SacI site , make sure you have the version from which the extra SacI site ...
Contents
BOOK | 1-2 |
DEVELOPMENT OF PLASMID CLONING VECTORS | 1-7 |
Index | 1-8 |
Copyright | |
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Common terms and phrases
agar agarose gel aliquots amber mutations bacteria bacteriophage M13 bacteriophage particles bacteriophage T4 bacteriophage T4 DNA BamHI Bgill buffer cDNA cells centrifugation at 12,000g cleaved cloning coli DNA polymerase colonies containing cosmid cosmid vector culture digestion DNA fragments DNA ligase DNA molecules DNA polymerase double-stranded DNA EcoRI EDTA efficiency Escherichia coli ethanol ethidium bromide eukaryotic eukaryotic DNA exonuclease extracts foreign DNA gel electrophoresis gene genetic Gp Cp HindIII Hindill host hybridization Incubate infected inserted intergenic region Kpnl lacZ linear lysogenic method methylase methylation microfuge tube minutes at 4°C nin5 nitrocellulose nucleotides origin of replication packaging pellet phagemids plaques plasmid plasmid DNA plate polycloning prepared protein Pvul recA recombinant red gam remove replication restriction enzyme room temperature Sacl Sall single-stranded DNA Smal solution sterile stored strains strand supernatant T4 DNA ligase teriophage Transfer Tris Cl pH vector DNA vitro volume Xbal µg/ml