Molecular Cloning: A Laboratory Manual, Volume 1 |
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Results 1-3 of 42
Page 5-33
... substrate that gives half - maximal activity ) . When using radioactively labeled substrates , calculate the concentration of substrate in the reaction from the specific activity provided by the manufacturer . If necessary , adjust the ...
... substrate that gives half - maximal activity ) . When using radioactively labeled substrates , calculate the concentration of substrate in the reaction from the specific activity provided by the manufacturer . If necessary , adjust the ...
Page 5-60
... substrate - dependent > 150 mM NaCl ( activity measured at nicks ) 10 % PEG , intermolecular ligation c 150-200 mM NaCl , blunt - end ligation stimulated by NaCl and KCI excess KCl inhibits on all substrates except single - stranded DNA ...
... substrate - dependent > 150 mM NaCl ( activity measured at nicks ) 10 % PEG , intermolecular ligation c 150-200 mM NaCl , blunt - end ligation stimulated by NaCl and KCI excess KCl inhibits on all substrates except single - stranded DNA ...
Page 5-63
... Substrate : Active on double - stranded DNA with complementary cohesive termini that base pair to bring together 3 ' - hydroxyl and 5 ' - phosphate termini . In addition , the enzyme is active on nicked DNA and active , albeit far less ...
... Substrate : Active on double - stranded DNA with complementary cohesive termini that base pair to bring together 3 ' - hydroxyl and 5 ' - phosphate termini . In addition , the enzyme is active on nicked DNA and active , albeit far less ...
Contents
BOOK | 1-2 |
DEVELOPMENT OF PLASMID CLONING VECTORS | 1-7 |
Index | 1-8 |
Copyright | |
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Common terms and phrases
agar agarose gel aliquots amber mutations bacteria bacteriophage M13 bacteriophage particles bacteriophage T4 bacteriophage T4 DNA BamHI Bgill buffer cDNA cells centrifugation at 12,000g cleaved cloning coli DNA polymerase colonies containing cosmid cosmid vector culture digestion DNA fragments DNA ligase DNA molecules DNA polymerase double-stranded DNA EcoRI EDTA efficiency Escherichia coli ethanol ethidium bromide eukaryotic eukaryotic DNA exonuclease extracts foreign DNA gel electrophoresis gene genetic Gp Cp HindIII Hindill host hybridization Incubate infected inserted intergenic region Kpnl lacZ linear lysogenic method methylase methylation microfuge tube minutes at 4°C nin5 nitrocellulose nucleotides origin of replication packaging pellet phagemids plaques plasmid plasmid DNA plate polycloning prepared protein Pvul recA recombinant red gam remove replication restriction enzyme room temperature Sacl Sall single-stranded DNA Smal solution sterile stored strains strand supernatant T4 DNA ligase teriophage Transfer Tris Cl pH vector DNA vitro volume Xbal µg/ml