Molecular Cloning: A Laboratory Manual, Volume 1 |
From inside the book
Results 1-3 of 10
Page 6-39
... Acrylamide acrylamide N , N ' - methylenebisacrylamide H2O to 100 ml 29 g 1 g Heat the solution to 37 ° C to dissolve the chemicals . Caution : Acrylamide is a potent neurotoxin and is absorbed through the skin . The effects of acrylamide ...
... Acrylamide acrylamide N , N ' - methylenebisacrylamide H2O to 100 ml 29 g 1 g Heat the solution to 37 ° C to dissolve the chemicals . Caution : Acrylamide is a potent neurotoxin and is absorbed through the skin . The effects of acrylamide ...
Page 6-41
... acrylamide solution from assembled gel molds , including : • Sealing the edges with agarose • { Inserting a plastic spacer into the open space at the bottom of the mold during polymerization • Sealing the bottom of the plate with a ...
... acrylamide solution from assembled gel molds , including : • Sealing the edges with agarose • { Inserting a plastic spacer into the open space at the bottom of the mold during polymerization • Sealing the bottom of the plate with a ...
Page 6-42
... acrylamide solution from the syringe , filling the space almost to the top . Keep the remaining acrylamide solution at 4 ° C to reduce the rate of polymerization . If the plates were clean , there should be no trapped air bubbles , and ...
... acrylamide solution from the syringe , filling the space almost to the top . Keep the remaining acrylamide solution at 4 ° C to reduce the rate of polymerization . If the plates were clean , there should be no trapped air bubbles , and ...
Contents
BOOK | 1-2 |
DEVELOPMENT OF PLASMID CLONING VECTORS | 1-7 |
Index | 1-8 |
Copyright | |
84 other sections not shown
Other editions - View all
Common terms and phrases
agar agarose gel aliquots amber mutations bacteria bacteriophage M13 bacteriophage particles bacteriophage T4 bacteriophage T4 DNA BamHI Bgill buffer cDNA cells centrifugation at 12,000g cleaved cloning coli DNA polymerase colonies containing cosmid cosmid vector culture digestion DNA fragments DNA ligase DNA molecules DNA polymerase double-stranded DNA EcoRI EDTA efficiency Escherichia coli ethanol ethidium bromide eukaryotic eukaryotic DNA exonuclease extracts foreign DNA gel electrophoresis gene genetic Gp Cp HindIII Hindill host hybridization Incubate infected inserted intergenic region Kpnl lacZ linear lysogenic method methylase methylation microfuge tube minutes at 4°C nin5 nitrocellulose nucleotides origin of replication packaging pellet phagemids plaques plasmid plasmid DNA plate polycloning prepared protein Pvul recA recombinant red gam remove replication restriction enzyme room temperature Sacl Sall single-stranded DNA Smal solution sterile stored strains strand supernatant T4 DNA ligase teriophage Transfer Tris Cl pH vector DNA vitro volume Xbal µg/ml