Molecular Cloning: A Laboratory Manual, Volume 1 |
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A Laboratory Manual Joseph Sambrook, E. F. Fritsch, Tom Maniatis. 6 Gel Electrophoresis of DNA Agarose Gel Electrophoresis 6.3 Factors Affecting the Rate of DNA Migration in Agarose Gels 6.4 MOLECULAR SIZE OF THE DNA 6.4 AGAROSE ...
A Laboratory Manual Joseph Sambrook, E. F. Fritsch, Tom Maniatis. 6 Gel Electrophoresis of DNA Agarose Gel Electrophoresis 6.3 Factors Affecting the Rate of DNA Migration in Agarose Gels 6.4 MOLECULAR SIZE OF THE DNA 6.4 AGAROSE ...
Page 6-2
... gel and used for a variety of cloning purposes . Agarose and polyacrylamide gels can be poured in a variety of shapes , sizes , and porosities and can be run in a number of different configurations . The choices within these parameters ...
... gel and used for a variety of cloning purposes . Agarose and polyacrylamide gels can be poured in a variety of shapes , sizes , and porosities and can be run in a number of different configurations . The choices within these parameters ...
Page 6-9
... AGAROSE GELS Preparation of an Agarose Gel 1. Seal the edges of a clean , dry , glass plate ( or the open ends of the plastic tray supplied with the electrophoresis ... AGAROSE GELS 6 9 Preparation of an Agarose Gel 6 9 STOCK SOLUTIONS B 9.
... AGAROSE GELS Preparation of an Agarose Gel 1. Seal the edges of a clean , dry , glass plate ( or the open ends of the plastic tray supplied with the electrophoresis ... AGAROSE GELS 6 9 Preparation of an Agarose Gel 6 9 STOCK SOLUTIONS B 9.
Contents
BOOK | 1-2 |
DEVELOPMENT OF PLASMID CLONING VECTORS | 1-7 |
Index | 1-8 |
Copyright | |
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agar agarose gel aliquots amber mutations bacteria bacteriophage M13 bacteriophage particles bacteriophage T4 bacteriophage T4 DNA BamHI Bgill buffer cDNA cells centrifugation at 12,000g cleaved cloning coli DNA polymerase colonies containing cosmid cosmid vector culture digestion DNA fragments DNA ligase DNA molecules DNA polymerase double-stranded DNA EcoRI EDTA efficiency Escherichia coli ethanol ethidium bromide eukaryotic eukaryotic DNA exonuclease extracts foreign DNA gel electrophoresis gene genetic Gp Cp HindIII Hindill host hybridization Incubate infected inserted intergenic region Kpnl lacZ linear lysogenic method methylase methylation microfuge tube minutes at 4°C nin5 nitrocellulose nucleotides origin of replication packaging pellet phagemids plaques plasmid plasmid DNA plate polycloning prepared protein Pvul recA recombinant red gam remove replication restriction enzyme room temperature Sacl Sall single-stranded DNA Smal solution sterile stored strains strand supernatant T4 DNA ligase teriophage Transfer Tris Cl pH vector DNA vitro volume Xbal µg/ml