Molecular Cloning: A Laboratory Manual, Volume 1 |
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Page 1-6
... amino - acid , membrane - associated protein ( Backman and Boyer 1983 ) that prevents the antibiotic from entering the cell ( Franklin 1967 ) . Ampicillin binds to and inhibits a number of enzymes in the bacterial membrane that are ...
... amino - acid , membrane - associated protein ( Backman and Boyer 1983 ) that prevents the antibiotic from entering the cell ( Franklin 1967 ) . Ampicillin binds to and inhibits a number of enzymes in the bacterial membrane that are ...
Page 2-47
... amino acid sequences introduced are Agt20 Glu GAA EcoRI Phe Ser Arg Glu Leu Val Asp Gin Phe TTC TCT AGA GAG CTC GTC GAC CAA TTC Xbal Sacl Sall Glu Leu Agt21 GAA TTG Val Asp GTC GAC Glu GAG Leu Ser Arg CTC TCT AGA Glu GAA TTC Phe Sall ...
... amino acid sequences introduced are Agt20 Glu GAA EcoRI Phe Ser Arg Glu Leu Val Asp Gin Phe TTC TCT AGA GAG CTC GTC GAC CAA TTC Xbal Sacl Sall Glu Leu Agt21 GAA TTG Val Asp GTC GAC Glu GAG Leu Ser Arg CTC TCT AGA Glu GAA TTC Phe Sall ...
Page 7-4
... amino acid sequence and predicted tertiary structure are similar to those of pancreatic RNAase ( Kurachi et al . 1985 ; Strydom et al . 1985 ) . The inhibitor , which is sold by several manufacturers under various trade names , should ...
... amino acid sequence and predicted tertiary structure are similar to those of pancreatic RNAase ( Kurachi et al . 1985 ; Strydom et al . 1985 ) . The inhibitor , which is sold by several manufacturers under various trade names , should ...
Contents
BOOK | 1-2 |
DEVELOPMENT OF PLASMID CLONING VECTORS | 1-7 |
Index | 1-8 |
Copyright | |
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agar agarose gel aliquots amber mutations bacteria bacteriophage M13 bacteriophage particles bacteriophage T4 bacteriophage T4 DNA BamHI Bgill buffer cDNA cells centrifugation at 12,000g cleaved cloning coli DNA polymerase colonies containing cosmid cosmid vector culture digestion DNA fragments DNA ligase DNA molecules DNA polymerase double-stranded DNA EcoRI EDTA efficiency Escherichia coli ethanol ethidium bromide eukaryotic eukaryotic DNA exonuclease extracts foreign DNA gel electrophoresis gene genetic Gp Cp HindIII Hindill host hybridization Incubate infected inserted intergenic region Kpnl lacZ linear lysogenic method methylase methylation microfuge tube minutes at 4°C nin5 nitrocellulose nucleotides origin of replication packaging pellet phagemids plaques plasmid plasmid DNA plate polycloning prepared protein Pvul recA recombinant red gam remove replication restriction enzyme room temperature Sacl Sall single-stranded DNA Smal solution sterile stored strains strand supernatant T4 DNA ligase teriophage Transfer Tris Cl pH vector DNA vitro volume Xbal µg/ml