Molecular Cloning: A Laboratory Manual, Volume 1 |
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Page xxxvii
... Amino Acids THE GENETIC CODE ( NUCLEAR GENES ) D.1 PROKARYOTIC SUPRESSORS OF NONSENSE MUTATIONS USED IN MOLECULAR CLONING D.1 PROPERTIES OF AMINO ACIDS D.2 Classification of Amino Acids D.6 Appendix E : Commonly Used Techniques in ...
... Amino Acids THE GENETIC CODE ( NUCLEAR GENES ) D.1 PROKARYOTIC SUPRESSORS OF NONSENSE MUTATIONS USED IN MOLECULAR CLONING D.1 PROPERTIES OF AMINO ACIDS D.2 Classification of Amino Acids D.6 Appendix E : Commonly Used Techniques in ...
Page 1-85
... amino acids of the ẞ - galactosidase gene ( lacZ ) . Embedded in this coding region is a polycloning site that does not disrupt the reading frame but results in the harmless interpolation of a small number of amino acids into the amino ...
... amino acids of the ẞ - galactosidase gene ( lacZ ) . Embedded in this coding region is a polycloning site that does not disrupt the reading frame but results in the harmless interpolation of a small number of amino acids into the amino ...
Page 2-57
... amino acids 11-41 . The amino - terminal fragments of ẞ - galac- tosidase produced by various vectors can associate with the lacZAM15 products to form enzymatically active proteins ( Beckwith 1964 ; Ullmann and Perrin 1970 ) . Bacterial ...
... amino acids 11-41 . The amino - terminal fragments of ẞ - galac- tosidase produced by various vectors can associate with the lacZAM15 products to form enzymatically active proteins ( Beckwith 1964 ; Ullmann and Perrin 1970 ) . Bacterial ...
Contents
BOOK | 1-2 |
DEVELOPMENT OF PLASMID CLONING VECTORS | 1-7 |
Index | 1-8 |
Copyright | |
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agar agarose gel aliquots amber mutations bacteria bacteriophage M13 bacteriophage particles bacteriophage T4 bacteriophage T4 DNA BamHI Bgill buffer cDNA cells centrifugation at 12,000g cleaved cloning coli DNA polymerase colonies containing cosmid cosmid vector culture digestion DNA fragments DNA ligase DNA molecules DNA polymerase double-stranded DNA EcoRI EDTA efficiency Escherichia coli ethanol ethidium bromide eukaryotic eukaryotic DNA exonuclease extracts foreign DNA gel electrophoresis gene genetic Gp Cp HindIII Hindill host hybridization Incubate infected inserted intergenic region Kpnl lacZ linear lysogenic method methylase methylation microfuge tube minutes at 4°C nin5 nitrocellulose nucleotides origin of replication packaging pellet phagemids plaques plasmid plasmid DNA plate polycloning prepared protein Pvul recA recombinant red gam remove replication restriction enzyme room temperature Sacl Sall single-stranded DNA Smal solution sterile stored strains strand supernatant T4 DNA ligase teriophage Transfer Tris Cl pH vector DNA vitro volume Xbal µg/ml