Molecular Cloning: A Laboratory Manual, Volume 1 |
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Page xxvii
... Antibodies in Immunological Screening 12.11 Choosing the Antibody 12.11 Purification of Antisera 12.13 Methods Used to Detect Antibodies Bound to Proteins Expressed in E. coli Validation of Clones Isolated by Immunological Screening ...
... Antibodies in Immunological Screening 12.11 Choosing the Antibody 12.11 Purification of Antisera 12.13 Methods Used to Detect Antibodies Bound to Proteins Expressed in E. coli Validation of Clones Isolated by Immunological Screening ...
Page xxxiv
... Antibodies 18.3 Factors Affecting Immune Response 18.3 SPECIES OF ANIMAL 18.3 GENETIC FACTORS 18.4 PHYSICAL STATE OF ... Antibodies 18.7 Raising Antisera Against Synthetic Peptides 18.7 COUPLING OF SYNTHETIC PEPTIDES TO KEYHOLE LIMPET ...
... Antibodies 18.3 Factors Affecting Immune Response 18.3 SPECIES OF ANIMAL 18.3 GENETIC FACTORS 18.4 PHYSICAL STATE OF ... Antibodies 18.7 Raising Antisera Against Synthetic Peptides 18.7 COUPLING OF SYNTHETIC PEPTIDES TO KEYHOLE LIMPET ...
Page xxxv
... Antibody Complexes 18.42 PRECLEARING THE CELL LYSATE 18.43 Immunoprecipitation of the Target Protein 18.44 SDS ... Antibody to the Target Protein 18.70 INCUBATING THE NITROCELLULOSE FILTER WITH THE PRIMARY ANTIBODY DIRECTED AGAINST THE ...
... Antibody Complexes 18.42 PRECLEARING THE CELL LYSATE 18.43 Immunoprecipitation of the Target Protein 18.44 SDS ... Antibody to the Target Protein 18.70 INCUBATING THE NITROCELLULOSE FILTER WITH THE PRIMARY ANTIBODY DIRECTED AGAINST THE ...
Contents
BOOK | 1-2 |
DEVELOPMENT OF PLASMID CLONING VECTORS | 1-7 |
Index | 1-8 |
Copyright | |
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agar agarose gel aliquots amber mutations bacteria bacteriophage M13 bacteriophage particles bacteriophage T4 bacteriophage T4 DNA BamHI Bgill buffer cDNA cells centrifugation at 12,000g cleaved cloning coli DNA polymerase colonies containing cosmid cosmid vector culture digestion DNA fragments DNA ligase DNA molecules DNA polymerase double-stranded DNA EcoRI EDTA efficiency Escherichia coli ethanol ethidium bromide eukaryotic eukaryotic DNA exonuclease extracts foreign DNA gel electrophoresis gene genetic Gp Cp HindIII Hindill host hybridization Incubate infected inserted intergenic region Kpnl lacZ linear lysogenic method methylase methylation microfuge tube minutes at 4°C nin5 nitrocellulose nucleotides origin of replication packaging pellet phagemids plaques plasmid plasmid DNA plate polycloning prepared protein Pvul recA recombinant red gam remove replication restriction enzyme room temperature Sacl Sall single-stranded DNA Smal solution sterile stored strains strand supernatant T4 DNA ligase teriophage Transfer Tris Cl pH vector DNA vitro volume Xbal µg/ml