Molecular Cloning: A Laboratory Manual, Volume 1 |
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Page 1-81
... appropriate volume ( up to 200 μl per 90 - mm plate ) of transformed competent cells onto agar SOB medium containing 20 mm MgSO , and the appropriate antibiotic . Add additional broth if small volumes of culture ( < 10 μl ) are ...
... appropriate volume ( up to 200 μl per 90 - mm plate ) of transformed competent cells onto agar SOB medium containing 20 mm MgSO , and the appropriate antibiotic . Add additional broth if small volumes of culture ( < 10 μl ) are ...
Page 2-83
... appropriate restriction enzyme buffer . Remove two aliquots ( 0.2 μg each ) of the undigested DNA and store on ice . 3. Add a threefold excess ( 75-150 units ) of the appropriate restriction enzyme and incubate for 1 hour at the optimal ...
... appropriate restriction enzyme buffer . Remove two aliquots ( 0.2 μg each ) of the undigested DNA and store on ice . 3. Add a threefold excess ( 75-150 units ) of the appropriate restriction enzyme and incubate for 1 hour at the optimal ...
Page 2-114
... Appropriate adjustments should be made to the volumes when carrying out hybridization reactions with different numbers or sizes of filters . 1. Float the baked filters on the surface of a tray of 2 × SSC until they have become ...
... Appropriate adjustments should be made to the volumes when carrying out hybridization reactions with different numbers or sizes of filters . 1. Float the baked filters on the surface of a tray of 2 × SSC until they have become ...
Contents
BOOK | 1-2 |
DEVELOPMENT OF PLASMID CLONING VECTORS | 1-7 |
Index | 1-8 |
Copyright | |
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Common terms and phrases
agar agarose gel aliquots amber mutations bacteria bacteriophage M13 bacteriophage particles bacteriophage T4 bacteriophage T4 DNA BamHI Bgill buffer cDNA cells centrifugation at 12,000g cleaved cloning coli DNA polymerase colonies containing cosmid cosmid vector culture digestion DNA fragments DNA ligase DNA molecules DNA polymerase double-stranded DNA EcoRI EDTA efficiency Escherichia coli ethanol ethidium bromide eukaryotic eukaryotic DNA exonuclease extracts foreign DNA gel electrophoresis gene genetic Gp Cp HindIII Hindill host hybridization Incubate infected inserted intergenic region Kpnl lacZ linear lysogenic method methylase methylation microfuge tube minutes at 4°C nin5 nitrocellulose nucleotides origin of replication packaging pellet phagemids plaques plasmid plasmid DNA plate polycloning prepared protein Pvul recA recombinant red gam remove replication restriction enzyme room temperature Sacl Sall single-stranded DNA Smal solution sterile stored strains strand supernatant T4 DNA ligase teriophage Transfer Tris Cl pH vector DNA vitro volume Xbal µg/ml