Molecular Cloning: A Laboratory Manual, Volume 1 |
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Page 1-21
... bacteria . These methods invariably involve three steps : • Growth of the bacterial culture Harvesting and lysis of the bacteria • Purification of plasmid DNA Growth of the Bacterial Culture Plasmids are almost always purified from ...
... bacteria . These methods invariably involve three steps : • Growth of the bacterial culture Harvesting and lysis of the bacteria • Purification of plasmid DNA Growth of the Bacterial Culture Plasmids are almost always purified from ...
Page 1-25
... Bacteria HARVESTING 1. Transfer a single bacterial colony into 2 ml of LB medium containing the appropriate antibiotic in a loosely capped 15 - ml tube . Incubate the culture overnight at 37 ° C with vigorous shaking . 2. Pour 1.5 ml of ...
... Bacteria HARVESTING 1. Transfer a single bacterial colony into 2 ml of LB medium containing the appropriate antibiotic in a loosely capped 15 - ml tube . Incubate the culture overnight at 37 ° C with vigorous shaking . 2. Pour 1.5 ml of ...
Page 1-33
... bacterial cultures that are allowed to grow to saturation . However , treatment with chloramphenicol has the advantage of inhibiting bacterial replication . This reduces the bulk and viscosity of the bacterial lysate and greatly ...
... bacterial cultures that are allowed to grow to saturation . However , treatment with chloramphenicol has the advantage of inhibiting bacterial replication . This reduces the bulk and viscosity of the bacterial lysate and greatly ...
Contents
BOOK | 1-2 |
DEVELOPMENT OF PLASMID CLONING VECTORS | 1-7 |
Index | 1-8 |
Copyright | |
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Common terms and phrases
agar agarose gel aliquots amber mutations bacteria bacteriophage M13 bacteriophage particles bacteriophage T4 bacteriophage T4 DNA BamHI Bgill buffer cDNA cells centrifugation at 12,000g cleaved cloning coli DNA polymerase colonies containing cosmid cosmid vector culture digestion DNA fragments DNA ligase DNA molecules DNA polymerase double-stranded DNA EcoRI EDTA efficiency Escherichia coli ethanol ethidium bromide eukaryotic eukaryotic DNA exonuclease extracts foreign DNA gel electrophoresis gene genetic Gp Cp HindIII Hindill host hybridization Incubate infected inserted intergenic region Kpnl lacZ linear lysogenic method methylase methylation microfuge tube minutes at 4°C nin5 nitrocellulose nucleotides origin of replication packaging pellet phagemids plaques plasmid plasmid DNA plate polycloning prepared protein Pvul recA recombinant red gam remove replication restriction enzyme room temperature Sacl Sall single-stranded DNA Smal solution sterile stored strains strand supernatant T4 DNA ligase teriophage Transfer Tris Cl pH vector DNA vitro volume Xbal µg/ml