Molecular Cloning: A Laboratory Manual, Volume 1 |
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Results 1-3 of 49
Page 1-68
... DNA . b . Add H2O to 7.5 μl , and warm the solution to 45 ° C for 5 minutes to melt any cohesive termini that have reannealed . Chill the mixture to 0 ° C . c . Add : 10 × bacteriophage T4 DNA ligase buffer bacteriophage T4 DNA ligase 5 ...
... DNA . b . Add H2O to 7.5 μl , and warm the solution to 45 ° C for 5 minutes to melt any cohesive termini that have reannealed . Chill the mixture to 0 ° C . c . Add : 10 × bacteriophage T4 DNA ligase buffer bacteriophage T4 DNA ligase 5 ...
Page 2-84
... bacteriophage T4 DNA ligase to the remain- der of the mixture , and incubate the reaction for 2 hours at 16 ° C . At least three different assays are used to measure the activity of bacteriophage T4 DNA ligase . Most manufacturers ...
... bacteriophage T4 DNA ligase to the remain- der of the mixture , and incubate the reaction for 2 hours at 16 ° C . At least three different assays are used to measure the activity of bacteriophage T4 DNA ligase . Most manufacturers ...
Page 5-62
A Laboratory Manual Joseph Sambrook, E. F. Fritsch, Tom Maniatis. Bacteriophage T4 DNA Ligase ( Bacteriophage T4 - infected E. coli ) USES r Bacteriophage T4 DNA ligase , a polypeptide of M1 = 68,000 , catalyzes the formation of ...
A Laboratory Manual Joseph Sambrook, E. F. Fritsch, Tom Maniatis. Bacteriophage T4 DNA Ligase ( Bacteriophage T4 - infected E. coli ) USES r Bacteriophage T4 DNA ligase , a polypeptide of M1 = 68,000 , catalyzes the formation of ...
Contents
BOOK | 1-2 |
DEVELOPMENT OF PLASMID CLONING VECTORS | 1-7 |
Index | 1-8 |
Copyright | |
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agar agarose gel aliquots amber mutations bacteria bacteriophage M13 bacteriophage particles bacteriophage T4 bacteriophage T4 DNA BamHI Bgill buffer cDNA cells centrifugation at 12,000g cleaved cloning coli DNA polymerase colonies containing cosmid cosmid vector culture digestion DNA fragments DNA ligase DNA molecules DNA polymerase double-stranded DNA EcoRI EDTA efficiency Escherichia coli ethanol ethidium bromide eukaryotic eukaryotic DNA exonuclease extracts foreign DNA gel electrophoresis gene genetic Gp Cp HindIII Hindill host hybridization Incubate infected inserted intergenic region Kpnl lacZ linear lysogenic method methylase methylation microfuge tube minutes at 4°C nin5 nitrocellulose nucleotides origin of replication packaging pellet phagemids plaques plasmid plasmid DNA plate polycloning prepared protein Pvul recA recombinant red gam remove replication restriction enzyme room temperature Sacl Sall single-stranded DNA Smal solution sterile stored strains strand supernatant T4 DNA ligase teriophage Transfer Tris Cl pH vector DNA vitro volume Xbal µg/ml