Molecular Cloning: A Laboratory Manual, Volume 1 |
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Page 2-73
A Laboratory Manual Joseph Sambrook, E. F. Fritsch, Tom Maniatis. À PURIFICATION OF BACTERIOPHAGE › The method given below for purification of bacteriophage particles is essen- tially that of Yamamoto et al . ( 1970 ) . Over the years ...
A Laboratory Manual Joseph Sambrook, E. F. Fritsch, Tom Maniatis. À PURIFICATION OF BACTERIOPHAGE › The method given below for purification of bacteriophage particles is essen- tially that of Yamamoto et al . ( 1970 ) . Over the years ...
Page 2-75
... particles . 12. Collect the bacteriophage particles by puncturing the side of the tube . Carefully wipe the outside of the tube with ethanol to remove any grease or oil , and then attach a piece of Scotch Tape to the outside of the tube ...
... particles . 12. Collect the bacteriophage particles by puncturing the side of the tube . Carefully wipe the outside of the tube with ethanol to remove any grease or oil , and then attach a piece of Scotch Tape to the outside of the tube ...
Page 2-77
... Bacteriophage λ PELLETING BACTERIOPHAGE PARTICLES 1. Follow the " Standard Method for Purification of Bacteriophage A " through step 8 ( page 2.73 ) , including extraction with chloroform . 2. Collect the bacteriophage particles by ...
... Bacteriophage λ PELLETING BACTERIOPHAGE PARTICLES 1. Follow the " Standard Method for Purification of Bacteriophage A " through step 8 ( page 2.73 ) , including extraction with chloroform . 2. Collect the bacteriophage particles by ...
Contents
BOOK | 1-2 |
DEVELOPMENT OF PLASMID CLONING VECTORS | 1-7 |
Index | 1-8 |
Copyright | |
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Common terms and phrases
agar agarose gel aliquots amber mutations bacteria bacteriophage M13 bacteriophage particles bacteriophage T4 bacteriophage T4 DNA BamHI Bgill buffer cDNA cells centrifugation at 12,000g cleaved cloning coli DNA polymerase colonies containing cosmid cosmid vector culture digestion DNA fragments DNA ligase DNA molecules DNA polymerase double-stranded DNA EcoRI EDTA efficiency Escherichia coli ethanol ethidium bromide eukaryotic eukaryotic DNA exonuclease extracts foreign DNA gel electrophoresis gene genetic Gp Cp HindIII Hindill host hybridization Incubate infected inserted intergenic region Kpnl lacZ linear lysogenic method methylase methylation microfuge tube minutes at 4°C nin5 nitrocellulose nucleotides origin of replication packaging pellet phagemids plaques plasmid plasmid DNA plate polycloning prepared protein Pvul recA recombinant red gam remove replication restriction enzyme room temperature Sacl Sall single-stranded DNA Smal solution sterile stored strains strand supernatant T4 DNA ligase teriophage Transfer Tris Cl pH vector DNA vitro volume Xbal µg/ml