Molecular Cloning: A Laboratory Manual, Volume 1 |
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Page 5-28
... buffer of lower ionic strength . The appropriate amount of NaCl and the second enzyme can then be added and the incubation continued . ( 2 ) A single buffer ( potassium glutamate buffer [ KGB ] ) can be used in which virtually all ...
... buffer of lower ionic strength . The appropriate amount of NaCl and the second enzyme can then be added and the incubation continued . ( 2 ) A single buffer ( potassium glutamate buffer [ KGB ] ) can be used in which virtually all ...
Page 7-27
... buffer . Apply the solution to the column , and immediately begin to collect the eluate in a sterile tube . When all of the RNA solution has entered the column , add 1 column volume of 1 × column - loading buffer and continue to collect ...
... buffer . Apply the solution to the column , and immediately begin to collect the eluate in a sterile tube . When all of the RNA solution has entered the column , add 1 column volume of 1 × column - loading buffer and continue to collect ...
Page 7-43
... buffer yellows with age if it is exposed to light or is autoclaved . Straw - colored buffer works well , but darker buffer does not . Caution : DEPC is suspected to be a carcinogen and should be handled with care . 2. Prepare the gel by ...
... buffer yellows with age if it is exposed to light or is autoclaved . Straw - colored buffer works well , but darker buffer does not . Caution : DEPC is suspected to be a carcinogen and should be handled with care . 2. Prepare the gel by ...
Contents
BOOK | 1-2 |
DEVELOPMENT OF PLASMID CLONING VECTORS | 1-7 |
Index | 1-8 |
Copyright | |
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Common terms and phrases
agar agarose gel aliquots amber mutations bacteria bacteriophage M13 bacteriophage particles bacteriophage T4 bacteriophage T4 DNA BamHI Bgill buffer cDNA cells centrifugation at 12,000g cleaved cloning coli DNA polymerase colonies containing cosmid cosmid vector culture digestion DNA fragments DNA ligase DNA molecules DNA polymerase double-stranded DNA EcoRI EDTA efficiency Escherichia coli ethanol ethidium bromide eukaryotic eukaryotic DNA exonuclease extracts foreign DNA gel electrophoresis gene genetic Gp Cp HindIII Hindill host hybridization Incubate infected inserted intergenic region Kpnl lacZ linear lysogenic method methylase methylation microfuge tube minutes at 4°C nin5 nitrocellulose nucleotides origin of replication packaging pellet phagemids plaques plasmid plasmid DNA plate polycloning prepared protein Pvul recA recombinant red gam remove replication restriction enzyme room temperature Sacl Sall single-stranded DNA Smal solution sterile stored strains strand supernatant T4 DNA ligase teriophage Transfer Tris Cl pH vector DNA vitro volume Xbal µg/ml