Molecular Cloning: A Laboratory Manual, Volume 1 |
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Results 1-3 of 78
Page 1-4
... carrying replicons whose components are not interchangeable belong to different groups . Over 30 such groups have now been recognized ( Datta 1979 ) . The replicons carried by plasmid vectors in current use are shown in Table 1.1 ...
... carrying replicons whose components are not interchangeable belong to different groups . Over 30 such groups have now been recognized ( Datta 1979 ) . The replicons carried by plasmid vectors in current use are shown in Table 1.1 ...
Page 1-6
... carried on plasmid pBR322 encodes a 399 - amino - acid , membrane - associated protein ( Backman and Boyer 1983 ) that prevents the antibiotic from entering the cell ( Franklin 1967 ) . Ampicillin binds to and inhibits a number of ...
... carried on plasmid pBR322 encodes a 399 - amino - acid , membrane - associated protein ( Backman and Boyer 1983 ) that prevents the antibiotic from entering the cell ( Franklin 1967 ) . Ampicillin binds to and inhibits a number of ...
Page 7-49
... carried out according to the instructions of the manufacturer of the apparatus that is used . Capillary elution is carried out essentially as described on pages 7.46-7.48 . Gels containing formaldehyde must be rinsed in several changes ...
... carried out according to the instructions of the manufacturer of the apparatus that is used . Capillary elution is carried out essentially as described on pages 7.46-7.48 . Gels containing formaldehyde must be rinsed in several changes ...
Contents
BOOK | 1-2 |
DEVELOPMENT OF PLASMID CLONING VECTORS | 1-7 |
Index | 1-8 |
Copyright | |
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Common terms and phrases
agar agarose gel aliquots amber mutations bacteria bacteriophage M13 bacteriophage particles bacteriophage T4 bacteriophage T4 DNA BamHI Bgill buffer cDNA cells centrifugation at 12,000g cleaved cloning coli DNA polymerase colonies containing cosmid cosmid vector culture digestion DNA fragments DNA ligase DNA molecules DNA polymerase double-stranded DNA EcoRI EDTA efficiency Escherichia coli ethanol ethidium bromide eukaryotic eukaryotic DNA exonuclease extracts foreign DNA gel electrophoresis gene genetic Gp Cp HindIII Hindill host hybridization Incubate infected inserted intergenic region Kpnl lacZ linear lysogenic method methylase methylation microfuge tube minutes at 4°C nin5 nitrocellulose nucleotides origin of replication packaging pellet phagemids plaques plasmid plasmid DNA plate polycloning prepared protein Pvul recA recombinant red gam remove replication restriction enzyme room temperature Sacl Sall single-stranded DNA Smal solution sterile stored strains strand supernatant T4 DNA ligase teriophage Transfer Tris Cl pH vector DNA vitro volume Xbal µg/ml