Molecular Cloning: A Laboratory Manual, Volume 1 |
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Results 1-3 of 77
Page 1-75
... cells ( Neumann et al . 1982 ; Potter et al . 1984 ; Fromm et al . 1985 ; Chu et al . 1987 ) , but it has recently been used to transform E. coli ( Dower et al . 1988 ; Taketo 1988 ) and other bacteria ( Chassy and Flickinger 1987 ...
... cells ( Neumann et al . 1982 ; Potter et al . 1984 ; Fromm et al . 1985 ; Chu et al . 1987 ) , but it has recently been used to transform E. coli ( Dower et al . 1988 ; Taketo 1988 ) and other bacteria ( Chassy and Flickinger 1987 ...
Page 1-80
... cells from the -70 ° C freezer . Thaw the cells by holding the tube in the palm of your hand . Just as the cells thaw , transfer the tube to an ice bath . Store the cells on ice for 10 minutes . v . Using a chilled , sterile pipette tip ...
... cells from the -70 ° C freezer . Thaw the cells by holding the tube in the palm of your hand . Just as the cells thaw , transfer the tube to an ice bath . Store the cells on ice for 10 minutes . v . Using a chilled , sterile pipette tip ...
Page 7-12
... Cells This is a convenient and well - tested procedure for purifying cytoplasmic RNA from cells grown in tissue culture . The method is similar to that described previously to isolate total cellular RNA , except that the nuclei are ...
... Cells This is a convenient and well - tested procedure for purifying cytoplasmic RNA from cells grown in tissue culture . The method is similar to that described previously to isolate total cellular RNA , except that the nuclei are ...
Contents
BOOK | 1-2 |
DEVELOPMENT OF PLASMID CLONING VECTORS | 1-7 |
Index | 1-8 |
Copyright | |
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Common terms and phrases
agar agarose gel aliquots amber mutations bacteria bacteriophage M13 bacteriophage particles bacteriophage T4 bacteriophage T4 DNA BamHI Bgill buffer cDNA cells centrifugation at 12,000g cleaved cloning coli DNA polymerase colonies containing cosmid cosmid vector culture digestion DNA fragments DNA ligase DNA molecules DNA polymerase double-stranded DNA EcoRI EDTA efficiency Escherichia coli ethanol ethidium bromide eukaryotic eukaryotic DNA exonuclease extracts foreign DNA gel electrophoresis gene genetic Gp Cp HindIII Hindill host hybridization Incubate infected inserted intergenic region Kpnl lacZ linear lysogenic method methylase methylation microfuge tube minutes at 4°C nin5 nitrocellulose nucleotides origin of replication packaging pellet phagemids plaques plasmid plasmid DNA plate polycloning prepared protein Pvul recA recombinant red gam remove replication restriction enzyme room temperature Sacl Sall single-stranded DNA Smal solution sterile stored strains strand supernatant T4 DNA ligase teriophage Transfer Tris Cl pH vector DNA vitro volume Xbal µg/ml