Molecular Cloning: A Laboratory Manual, Volume 1 |
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Results 1-3 of 32
Page 1-40
... centrifugation at 12,000g for 5 minutes at 4 ° C in a microfuge . 6. Remove the supernatant by aspiration . Dissolve the pellet of plasmid DNA in 400 μl of TE ( pH 8.0 ) . Extract the solution once with phenol , once with phenol ...
... centrifugation at 12,000g for 5 minutes at 4 ° C in a microfuge . 6. Remove the supernatant by aspiration . Dissolve the pellet of plasmid DNA in 400 μl of TE ( pH 8.0 ) . Extract the solution once with phenol , once with phenol ...
Page 4-32
... centrifugation at 12,000g for 20 minutes at 4 ° C . 10. Carefully discard all of the supernatant . Add 30 ml of 70 % ethanol at 4 ° C to the tube . Vortex briefly . 11. Centrifuge at 12,000g for 10 minutes at 4 ° C . Carefully remove as ...
... centrifugation at 12,000g for 20 minutes at 4 ° C . 10. Carefully discard all of the supernatant . Add 30 ml of 70 % ethanol at 4 ° C to the tube . Vortex briefly . 11. Centrifuge at 12,000g for 10 minutes at 4 ° C . Carefully remove as ...
Page 7-14
... centrifugation at 12,000g for 5 minutes at room temperature in a microfuge . Transfer the aqueous phase to a fresh microfuge tube , and add 3 M sodium acetate ( pH 5.2 ) to a final concentration of 0.3 M. Add 2.5 volumes of ice - cold ...
... centrifugation at 12,000g for 5 minutes at room temperature in a microfuge . Transfer the aqueous phase to a fresh microfuge tube , and add 3 M sodium acetate ( pH 5.2 ) to a final concentration of 0.3 M. Add 2.5 volumes of ice - cold ...
Contents
BOOK | 1-2 |
DEVELOPMENT OF PLASMID CLONING VECTORS | 1-7 |
Index | 1-8 |
Copyright | |
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agar agarose gel aliquots amber mutations bacteria bacteriophage M13 bacteriophage particles bacteriophage T4 bacteriophage T4 DNA BamHI Bgill buffer cDNA cells centrifugation at 12,000g cleaved cloning coli DNA polymerase colonies containing cosmid cosmid vector culture digestion DNA fragments DNA ligase DNA molecules DNA polymerase double-stranded DNA EcoRI EDTA efficiency Escherichia coli ethanol ethidium bromide eukaryotic eukaryotic DNA exonuclease extracts foreign DNA gel electrophoresis gene genetic Gp Cp HindIII Hindill host hybridization Incubate infected inserted intergenic region Kpnl lacZ linear lysogenic method methylase methylation microfuge tube minutes at 4°C nin5 nitrocellulose nucleotides origin of replication packaging pellet phagemids plaques plasmid plasmid DNA plate polycloning prepared protein Pvul recA recombinant red gam remove replication restriction enzyme room temperature Sacl Sall single-stranded DNA Smal solution sterile stored strains strand supernatant T4 DNA ligase teriophage Transfer Tris Cl pH vector DNA vitro volume Xbal µg/ml