Molecular Cloning: A Laboratory Manual, Volume 1 |
From inside the book
Results 1-3 of 42
Page xxix
... Cleavage at G residues 13.88 Cleavage at Purine Residues ( AG ) 13.90 Cleavage at Pyrimidine Residues ( C + T ) 13.91 Cleavage at C Residues 13.92 Cleavage at A and C Residues ( A > C ) 13.93 Treatment of Samples with Piperidine 13.94 ...
... Cleavage at G residues 13.88 Cleavage at Purine Residues ( AG ) 13.90 Cleavage at Pyrimidine Residues ( C + T ) 13.91 Cleavage at C Residues 13.92 Cleavage at A and C Residues ( A > C ) 13.93 Treatment of Samples with Piperidine 13.94 ...
Page 1-28
... cleavage with restriction enzyme ( s ) , remove 1 μl of the DNA solution and add it to a fresh microfuge tube that contains 8 μl of water . Add 1 μl of the appropriate 10 x restriction enzyme buffer and 1 unit of the desired restriction ...
... cleavage with restriction enzyme ( s ) , remove 1 μl of the DNA solution and add it to a fresh microfuge tube that contains 8 μl of water . Add 1 μl of the appropriate 10 x restriction enzyme buffer and 1 unit of the desired restriction ...
Page 5-8
... cleavage are indicated in parentheses . For example , HgaI GACGC ( 5/10 ) indicates cleavage as shown below : 5 ' GACGCNNNNN ↓ 3 ' 3 ' CTGCGNNNNNNNNNN ↓ 5 ' In some of these cases , the actual site of cleavage varies slightly ...
... cleavage are indicated in parentheses . For example , HgaI GACGC ( 5/10 ) indicates cleavage as shown below : 5 ' GACGCNNNNN ↓ 3 ' 3 ' CTGCGNNNNNNNNNN ↓ 5 ' In some of these cases , the actual site of cleavage varies slightly ...
Contents
BOOK | 1-2 |
DEVELOPMENT OF PLASMID CLONING VECTORS | 1-7 |
Index | 1-8 |
Copyright | |
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agar agarose gel aliquots amber mutations bacteria bacteriophage M13 bacteriophage particles bacteriophage T4 bacteriophage T4 DNA BamHI Bgill buffer cDNA cells centrifugation at 12,000g cleaved cloning coli DNA polymerase colonies containing cosmid cosmid vector culture digestion DNA fragments DNA ligase DNA molecules DNA polymerase double-stranded DNA EcoRI EDTA efficiency Escherichia coli ethanol ethidium bromide eukaryotic eukaryotic DNA exonuclease extracts foreign DNA gel electrophoresis gene genetic Gp Cp HindIII Hindill host hybridization Incubate infected inserted intergenic region Kpnl lacZ linear lysogenic method methylase methylation microfuge tube minutes at 4°C nin5 nitrocellulose nucleotides origin of replication packaging pellet phagemids plaques plasmid plasmid DNA plate polycloning prepared protein Pvul recA recombinant red gam remove replication restriction enzyme room temperature Sacl Sall single-stranded DNA Smal solution sterile stored strains strand supernatant T4 DNA ligase teriophage Transfer Tris Cl pH vector DNA vitro volume Xbal µg/ml