Molecular Cloning: A Laboratory Manual, Volume 1 |
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Page xxxiii
... Cleaved by a Protease or Cyanogen Bromide 17.27 PRODUCTION OF HYBRID PROTEINS THAT CAN BE CLEAVED WITH FACTOR X 17.28 Expression of Secreted Foreign Proteins 17.31 phoA - MEDIATED EXPRESSION AND SECRETION 17.32 a QUANTITATING THE LEVELS ...
... Cleaved by a Protease or Cyanogen Bromide 17.27 PRODUCTION OF HYBRID PROTEINS THAT CAN BE CLEAVED WITH FACTOR X 17.28 Expression of Secreted Foreign Proteins 17.31 phoA - MEDIATED EXPRESSION AND SECRETION 17.32 a QUANTITATING THE LEVELS ...
Page 1-66
... cleaved with two different restriction enzymes ; stuffer fragment not removed . ( ) Vector cleaved with one restriction enzyme and dephosphorylated . ( △ ) Vector cleaved with one restriction enzyme and not dephosphorylated . This ...
... cleaved with two different restriction enzymes ; stuffer fragment not removed . ( ) Vector cleaved with one restriction enzyme and dephosphorylated . ( △ ) Vector cleaved with one restriction enzyme and not dephosphorylated . This ...
Page 5-14
... cleaved with BamHI . ( Note : Only 1 in every 16 MboI / BamHI fusions regenerate a BamHI site , and therefore it is not usually possible to recover an Mbol fragment inserted into a BamHI vector by digesting the recombinant with BamHI ...
... cleaved with BamHI . ( Note : Only 1 in every 16 MboI / BamHI fusions regenerate a BamHI site , and therefore it is not usually possible to recover an Mbol fragment inserted into a BamHI vector by digesting the recombinant with BamHI ...
Contents
BOOK | 1-2 |
DEVELOPMENT OF PLASMID CLONING VECTORS | 1-7 |
Index | 1-8 |
Copyright | |
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agar agarose gel aliquots amber mutations bacteria bacteriophage M13 bacteriophage particles bacteriophage T4 bacteriophage T4 DNA BamHI Bgill buffer cDNA cells centrifugation at 12,000g cleaved cloning coli DNA polymerase colonies containing cosmid cosmid vector culture digestion DNA fragments DNA ligase DNA molecules DNA polymerase double-stranded DNA EcoRI EDTA efficiency Escherichia coli ethanol ethidium bromide eukaryotic eukaryotic DNA exonuclease extracts foreign DNA gel electrophoresis gene genetic Gp Cp HindIII Hindill host hybridization Incubate infected inserted intergenic region Kpnl lacZ linear lysogenic method methylase methylation microfuge tube minutes at 4°C nin5 nitrocellulose nucleotides origin of replication packaging pellet phagemids plaques plasmid plasmid DNA plate polycloning prepared protein Pvul recA recombinant red gam remove replication restriction enzyme room temperature Sacl Sall single-stranded DNA Smal solution sterile stored strains strand supernatant T4 DNA ligase teriophage Transfer Tris Cl pH vector DNA vitro volume Xbal µg/ml