Molecular Cloning: A Laboratory Manual, Volume 1 |
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Page 1-90
... colonies carrying cosmids or plasmids that contain DNA sequences of interest . Methods are presented below to screen bacterial colonies with radiolabeled probes that are longer than 100 nucleotides in length . The preparation of these ...
... colonies carrying cosmids or plasmids that contain DNA sequences of interest . Methods are presented below to screen bacterial colonies with radiolabeled probes that are longer than 100 nucleotides in length . The preparation of these ...
Page 1-92
... Colonies to Nitrocellulose Filters This procedure is used when it is necessary to screen a small number of bacterial colonies ( 100-200 ) that are dispersed over several agar plates . The colonies are consolidated on a master agar plate ...
... Colonies to Nitrocellulose Filters This procedure is used when it is necessary to screen a small number of bacterial colonies ( 100-200 ) that are dispersed over several agar plates . The colonies are consolidated on a master agar plate ...
Page 4-48
... colonies , i.e. , colonies that have been grown for 18 hours or less and have not been stored at 4 ° C . Older colonies or colonies that have been stored at 4 ° C should be treated as follows : 1. Inoculate 2 ml of 2 × YT medium ...
... colonies , i.e. , colonies that have been grown for 18 hours or less and have not been stored at 4 ° C . Older colonies or colonies that have been stored at 4 ° C should be treated as follows : 1. Inoculate 2 ml of 2 × YT medium ...
Contents
BOOK | 1-2 |
DEVELOPMENT OF PLASMID CLONING VECTORS | 1-7 |
Index | 1-8 |
Copyright | |
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Common terms and phrases
agar agarose gel aliquots amber mutations bacteria bacteriophage M13 bacteriophage particles bacteriophage T4 bacteriophage T4 DNA BamHI Bgill buffer cDNA cells centrifugation at 12,000g cleaved cloning coli DNA polymerase colonies containing cosmid cosmid vector culture digestion DNA fragments DNA ligase DNA molecules DNA polymerase double-stranded DNA EcoRI EDTA efficiency Escherichia coli ethanol ethidium bromide eukaryotic eukaryotic DNA exonuclease extracts foreign DNA gel electrophoresis gene genetic Gp Cp HindIII Hindill host hybridization Incubate infected inserted intergenic region Kpnl lacZ linear lysogenic method methylase methylation microfuge tube minutes at 4°C nin5 nitrocellulose nucleotides origin of replication packaging pellet phagemids plaques plasmid plasmid DNA plate polycloning prepared protein Pvul recA recombinant red gam remove replication restriction enzyme room temperature Sacl Sall single-stranded DNA Smal solution sterile stored strains strand supernatant T4 DNA ligase teriophage Transfer Tris Cl pH vector DNA vitro volume Xbal µg/ml