Molecular Cloning: A Laboratory Manual, Volume 1 |
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Page 1-64
... concentration of the DNA . Theoretically , when j = i , the end of a given DNA molecule is equally likely to make contact either with the other end of the same molecule or the end of a different molecule . Thus , under these conditions ...
... concentration of the DNA . Theoretically , when j = i , the end of a given DNA molecule is equally likely to make contact either with the other end of the same molecule or the end of a different molecule . Thus , under these conditions ...
Page 7-8
... concentration of 1000 units / ml or 10 mм , respectively . 7. Add RNAase - free pancreatic DNAase I ( see Appendix B ) to a final concentration of 2 μg / ml . Incubate the mixture for 60 minutes at 37 ° C . 8. Add EDTA and SDS to final ...
... concentration of 1000 units / ml or 10 mм , respectively . 7. Add RNAase - free pancreatic DNAase I ( see Appendix B ) to a final concentration of 2 μg / ml . Incubate the mixture for 60 minutes at 37 ° C . 8. Add EDTA and SDS to final ...
Page 7-14
... concentration of 1000 units / ml or 10 mм , respectively . 12. Add RNAase - free pancreatic DNAase I ( see Appendix B ) to a final concentration of 2 μg / ml . Incubate for 60 minutes at 37 ° C . 13. Add EDTA and SDS to final concentrations ...
... concentration of 1000 units / ml or 10 mм , respectively . 12. Add RNAase - free pancreatic DNAase I ( see Appendix B ) to a final concentration of 2 μg / ml . Incubate for 60 minutes at 37 ° C . 13. Add EDTA and SDS to final concentrations ...
Contents
BOOK | 1-2 |
DEVELOPMENT OF PLASMID CLONING VECTORS | 1-7 |
Index | 1-8 |
Copyright | |
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Common terms and phrases
agar agarose gel aliquots amber mutations bacteria bacteriophage M13 bacteriophage particles bacteriophage T4 bacteriophage T4 DNA BamHI Bgill buffer cDNA cells centrifugation at 12,000g cleaved cloning coli DNA polymerase colonies containing cosmid cosmid vector culture digestion DNA fragments DNA ligase DNA molecules DNA polymerase double-stranded DNA EcoRI EDTA efficiency Escherichia coli ethanol ethidium bromide eukaryotic eukaryotic DNA exonuclease extracts foreign DNA gel electrophoresis gene genetic Gp Cp HindIII Hindill host hybridization Incubate infected inserted intergenic region Kpnl lacZ linear lysogenic method methylase methylation microfuge tube minutes at 4°C nin5 nitrocellulose nucleotides origin of replication packaging pellet phagemids plaques plasmid plasmid DNA plate polycloning prepared protein Pvul recA recombinant red gam remove replication restriction enzyme room temperature Sacl Sall single-stranded DNA Smal solution sterile stored strains strand supernatant T4 DNA ligase teriophage Transfer Tris Cl pH vector DNA vitro volume Xbal µg/ml