Molecular Cloning: A Laboratory Manual, Volume 1 |
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Results 1-3 of 82
Page 2-94
... contain 1 μg of bacteriophage A arms , 40 kb in size . In ligation mixtures containing the lowest recommended amount of potential insert , the bac- teriophage arms will be present in eightfold molar excess ; in mixtures containing the ...
... contain 1 μg of bacteriophage A arms , 40 kb in size . In ligation mixtures containing the lowest recommended amount of potential insert , the bac- teriophage arms will be present in eightfold molar excess ; in mixtures containing the ...
Page 4-48
... containing pUC118 or pUC119 or their derivatives in a sterile 15 - ml culture tube containing 2-3 ml of 2 × YT medium . Add M13KO7 to a final concentration of 2 × 107 pfu / ml . Incu- bate for 1.0-1.5 hours at 37 ° C with strong ...
... containing pUC118 or pUC119 or their derivatives in a sterile 15 - ml culture tube containing 2-3 ml of 2 × YT medium . Add M13KO7 to a final concentration of 2 × 107 pfu / ml . Incu- bate for 1.0-1.5 hours at 37 ° C with strong ...
Page 7-43
... Containing Formaldehyde This method is adapted from those of Lehrach et al . ( 1977 ) , Goldberg ( 1980 ) , and Seed ( 1982a ) . Caution : Formaldehyde vapors are toxic . Solutions containing formal- dehyde should be prepared in a ...
... Containing Formaldehyde This method is adapted from those of Lehrach et al . ( 1977 ) , Goldberg ( 1980 ) , and Seed ( 1982a ) . Caution : Formaldehyde vapors are toxic . Solutions containing formal- dehyde should be prepared in a ...
Contents
BOOK | 1-2 |
DEVELOPMENT OF PLASMID CLONING VECTORS | 1-7 |
Index | 1-8 |
Copyright | |
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Common terms and phrases
agar agarose gel aliquots amber mutations bacteria bacteriophage M13 bacteriophage particles bacteriophage T4 bacteriophage T4 DNA BamHI Bgill buffer cDNA cells centrifugation at 12,000g cleaved cloning coli DNA polymerase colonies containing cosmid cosmid vector culture digestion DNA fragments DNA ligase DNA molecules DNA polymerase double-stranded DNA EcoRI EDTA efficiency Escherichia coli ethanol ethidium bromide eukaryotic eukaryotic DNA exonuclease extracts foreign DNA gel electrophoresis gene genetic Gp Cp HindIII Hindill host hybridization Incubate infected inserted intergenic region Kpnl lacZ linear lysogenic method methylase methylation microfuge tube minutes at 4°C nin5 nitrocellulose nucleotides origin of replication packaging pellet phagemids plaques plasmid plasmid DNA plate polycloning prepared protein Pvul recA recombinant red gam remove replication restriction enzyme room temperature Sacl Sall single-stranded DNA Smal solution sterile stored strains strand supernatant T4 DNA ligase teriophage Transfer Tris Cl pH vector DNA vitro volume Xbal µg/ml