Molecular Cloning: A Laboratory Manual, Volume 1 |
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Page 3-5
A Laboratory Manual Joseph Sambrook, E. F. Fritsch, Tom Maniatis. Cloning in Cosmid Vectors In their simplest form , cosmid vectors are modified plasmids that carry a copy of the DNA sequences ( cos sequences ) required for packaging DNA ...
A Laboratory Manual Joseph Sambrook, E. F. Fritsch, Tom Maniatis. Cloning in Cosmid Vectors In their simplest form , cosmid vectors are modified plasmids that carry a copy of the DNA sequences ( cos sequences ) required for packaging DNA ...
Page 3-7
... cosmid vector ( Bates and Swift 1983 ; Poustka et al . 1984 ) . As discussed later , the presence of these two sites greatly simplifies directional cloning in cosmid vectors ( see Figure 3.3 ) . • The construction of cosmid vectors that ...
... cosmid vector ( Bates and Swift 1983 ; Poustka et al . 1984 ) . As discussed later , the presence of these two sites greatly simplifies directional cloning in cosmid vectors ( see Figure 3.3 ) . • The construction of cosmid vectors that ...
Page 3-18
... cosmids that carry selectable markers for eukaryotic cells are de- scribed on the following pages . pHC79-2cos / tk + pHC79-2cos / tk ( Lindenmaier et 3.18 Cosmid Vectors COSMID VECTORS FOR TRANSFECTION OF MAMMALIAN CELLS 3 18.
... cosmids that carry selectable markers for eukaryotic cells are de- scribed on the following pages . pHC79-2cos / tk + pHC79-2cos / tk ( Lindenmaier et 3.18 Cosmid Vectors COSMID VECTORS FOR TRANSFECTION OF MAMMALIAN CELLS 3 18.
Contents
BOOK | 1-2 |
DEVELOPMENT OF PLASMID CLONING VECTORS | 1-7 |
Index | 1-8 |
Copyright | |
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agar agarose gel aliquots amber mutations bacteria bacteriophage M13 bacteriophage particles bacteriophage T4 bacteriophage T4 DNA BamHI Bgill buffer cDNA cells centrifugation at 12,000g cleaved cloning coli DNA polymerase colonies containing cosmid cosmid vector culture digestion DNA fragments DNA ligase DNA molecules DNA polymerase double-stranded DNA EcoRI EDTA efficiency Escherichia coli ethanol ethidium bromide eukaryotic eukaryotic DNA exonuclease extracts foreign DNA gel electrophoresis gene genetic Gp Cp HindIII Hindill host hybridization Incubate infected inserted intergenic region Kpnl lacZ linear lysogenic method methylase methylation microfuge tube minutes at 4°C nin5 nitrocellulose nucleotides origin of replication packaging pellet phagemids plaques plasmid plasmid DNA plate polycloning prepared protein Pvul recA recombinant red gam remove replication restriction enzyme room temperature Sacl Sall single-stranded DNA Smal solution sterile stored strains strand supernatant T4 DNA ligase teriophage Transfer Tris Cl pH vector DNA vitro volume Xbal µg/ml