Molecular Cloning: A Laboratory Manual, Volume 1 |
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Results 1-3 of 28
Page xxx
... Deletions and Insertions 15.3 SIMPLE DELETIONS OR INSERTIONS 15.3 SYSTEMATIC DELETIONS AND INSERTIONS 15.5 Linker - insertion Mutagenesis 15.5 GENERATION OF LINKER - INSERTION MUTANTS WITH FREQUENTLY CUTTING RESTRICTION ENZYMES 15.8 ...
... Deletions and Insertions 15.3 SIMPLE DELETIONS OR INSERTIONS 15.3 SYSTEMATIC DELETIONS AND INSERTIONS 15.5 Linker - insertion Mutagenesis 15.5 GENERATION OF LINKER - INSERTION MUTANTS WITH FREQUENTLY CUTTING RESTRICTION ENZYMES 15.8 ...
Page 2-16
... Deletions of the b region that damage the att site and therefore prevent lysogenization . int 29 b2 b1007 6527 b189 KH52 A deletion in bacteriophage 480 DNA . KH53 KH54 nin5 nin L44 WL113 ss / IA1-2 shndIII ^ 2-3 sx IA1 ° Mouse stuffer ...
... Deletions of the b region that damage the att site and therefore prevent lysogenization . int 29 b2 b1007 6527 b189 KH52 A deletion in bacteriophage 480 DNA . KH53 KH54 nin5 nin L44 WL113 ss / IA1-2 shndIII ^ 2-3 sx IA1 ° Mouse stuffer ...
Page 2-25
... deletion WL113 , which extends from bacteriophage A SalI site 2 ( 33,240 ) to bacteriophage A BamHI site 4 ( 34,500 ) ... Deletions Substitutions Insertions red / gam status of vector red / gam status of recombinants chi site present in ...
... deletion WL113 , which extends from bacteriophage A SalI site 2 ( 33,240 ) to bacteriophage A BamHI site 4 ( 34,500 ) ... Deletions Substitutions Insertions red / gam status of vector red / gam status of recombinants chi site present in ...
Contents
BOOK | 1-2 |
DEVELOPMENT OF PLASMID CLONING VECTORS | 1-7 |
Index | 1-8 |
Copyright | |
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agar agarose gel aliquots amber mutations bacteria bacteriophage M13 bacteriophage particles bacteriophage T4 bacteriophage T4 DNA BamHI Bgill buffer cDNA cells centrifugation at 12,000g cleaved cloning coli DNA polymerase colonies containing cosmid cosmid vector culture digestion DNA fragments DNA ligase DNA molecules DNA polymerase double-stranded DNA EcoRI EDTA efficiency Escherichia coli ethanol ethidium bromide eukaryotic eukaryotic DNA exonuclease extracts foreign DNA gel electrophoresis gene genetic Gp Cp HindIII Hindill host hybridization Incubate infected inserted intergenic region Kpnl lacZ linear lysogenic method methylase methylation microfuge tube minutes at 4°C nin5 nitrocellulose nucleotides origin of replication packaging pellet phagemids plaques plasmid plasmid DNA plate polycloning prepared protein Pvul recA recombinant red gam remove replication restriction enzyme room temperature Sacl Sall single-stranded DNA Smal solution sterile stored strains strand supernatant T4 DNA ligase teriophage Transfer Tris Cl pH vector DNA vitro volume Xbal µg/ml