Molecular Cloning: A Laboratory Manual, Volume 1 |
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Page 1-74
... efficiency of transformation of different bacterial strains by plasmids . Bac- teria treated according to the original protocol of Mandel and Higa yield 105-106 transformed colonies / μg of supercoiled plasmid DNA . This efficiency can ...
... efficiency of transformation of different bacterial strains by plasmids . Bac- teria treated according to the original protocol of Mandel and Higa yield 105-106 transformed colonies / μg of supercoiled plasmid DNA . This efficiency can ...
Page 1-75
... efficiencies . The efficiency of transformation with linear bacteriophage λ DNA molecules is only 0.1 % of that obtained with small supercoiled plasmid DNA . A two- to tenfold variation in transforma- tion efficiency has been observed ...
... efficiencies . The efficiency of transformation with linear bacteriophage λ DNA molecules is only 0.1 % of that obtained with small supercoiled plasmid DNA . A two- to tenfold variation in transforma- tion efficiency has been observed ...
Page 1-76
... efficiency of bacterial transformation , it is best to set aside a batch of glassware that is used for no other purpose than to prepare competent bacteria . This glassware should be washed and rinsed by hand , filled with pure water ...
... efficiency of bacterial transformation , it is best to set aside a batch of glassware that is used for no other purpose than to prepare competent bacteria . This glassware should be washed and rinsed by hand , filled with pure water ...
Contents
BOOK | 1-2 |
DEVELOPMENT OF PLASMID CLONING VECTORS | 1-7 |
Index | 1-8 |
Copyright | |
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Common terms and phrases
agar agarose gel aliquots amber mutations bacteria bacteriophage M13 bacteriophage particles bacteriophage T4 bacteriophage T4 DNA BamHI Bgill buffer cDNA cells centrifugation at 12,000g cleaved cloning coli DNA polymerase colonies containing cosmid cosmid vector culture digestion DNA fragments DNA ligase DNA molecules DNA polymerase double-stranded DNA EcoRI EDTA efficiency Escherichia coli ethanol ethidium bromide eukaryotic eukaryotic DNA exonuclease extracts foreign DNA gel electrophoresis gene genetic Gp Cp HindIII Hindill host hybridization Incubate infected inserted intergenic region Kpnl lacZ linear lysogenic method methylase methylation microfuge tube minutes at 4°C nin5 nitrocellulose nucleotides origin of replication packaging pellet phagemids plaques plasmid plasmid DNA plate polycloning prepared protein Pvul recA recombinant red gam remove replication restriction enzyme room temperature Sacl Sall single-stranded DNA Smal solution sterile stored strains strand supernatant T4 DNA ligase teriophage Transfer Tris Cl pH vector DNA vitro volume Xbal µg/ml