Molecular Cloning: A Laboratory Manual, Volume 1 |
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Results 1-3 of 69
Page 6-26
... ethanol at 4 ° C . Store the mixture for 10 minutes at room temperature , and recover the DNA by centrifugation at 12,000g for 20 minutes at room temperature in a microfuge . Carefully rinse the pellet with 70 % ethanol , and then ...
... ethanol at 4 ° C . Store the mixture for 10 minutes at room temperature , and recover the DNA by centrifugation at 12,000g for 20 minutes at room temperature in a microfuge . Carefully rinse the pellet with 70 % ethanol , and then ...
Page 7-8
... ethanol . Stand the open tube on the bench for a few minutes to allow the last traces of ethanol to evaporate . 13. Redissolve the pellet in 200 μl of TE ( pH 7.6 ) . Add 500 μl of ethanol , and store the preparation at -70 ° C until it ...
... ethanol . Stand the open tube on the bench for a few minutes to allow the last traces of ethanol to evaporate . 13. Redissolve the pellet in 200 μl of TE ( pH 7.6 ) . Add 500 μl of ethanol , and store the preparation at -70 ° C until it ...
Page 7-82
... ethanol at a concentration of 5 mg / ml , stored at −20 ° C in the dark , and diluted into the reaction mixture ... ethanol . Mix well , and store at 0 ° C for 1 hour . 11. Recover the nucleic acids by centrifugation at 12,000g for 15 ...
... ethanol at a concentration of 5 mg / ml , stored at −20 ° C in the dark , and diluted into the reaction mixture ... ethanol . Mix well , and store at 0 ° C for 1 hour . 11. Recover the nucleic acids by centrifugation at 12,000g for 15 ...
Contents
BOOK | 1-2 |
DEVELOPMENT OF PLASMID CLONING VECTORS | 1-7 |
Index | 1-8 |
Copyright | |
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Common terms and phrases
agar agarose gel aliquots amber mutations bacteria bacteriophage M13 bacteriophage particles bacteriophage T4 bacteriophage T4 DNA BamHI Bgill buffer cDNA cells centrifugation at 12,000g cleaved cloning coli DNA polymerase colonies containing cosmid cosmid vector culture digestion DNA fragments DNA ligase DNA molecules DNA polymerase double-stranded DNA EcoRI EDTA efficiency Escherichia coli ethanol ethidium bromide eukaryotic eukaryotic DNA exonuclease extracts foreign DNA gel electrophoresis gene genetic Gp Cp HindIII Hindill host hybridization Incubate infected inserted intergenic region Kpnl lacZ linear lysogenic method methylase methylation microfuge tube minutes at 4°C nin5 nitrocellulose nucleotides origin of replication packaging pellet phagemids plaques plasmid plasmid DNA plate polycloning prepared protein Pvul recA recombinant red gam remove replication restriction enzyme room temperature Sacl Sall single-stranded DNA Smal solution sterile stored strains strand supernatant T4 DNA ligase teriophage Transfer Tris Cl pH vector DNA vitro volume Xbal µg/ml