Molecular Cloning: A Laboratory Manual, Volume 1 |
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Page 5-40
... exonuclease activity can be removed proteolytically from the holoenzyme without affecting either the polymerase activity or the 3 ' 5 ' exonuclease activity ( Klenow and Henningsen 1970 ) . The Klenow fragment of E. coli DNA polymerase ...
... exonuclease activity can be removed proteolytically from the holoenzyme without affecting either the polymerase activity or the 3 ' 5 ' exonuclease activity ( Klenow and Henningsen 1970 ) . The Klenow fragment of E. coli DNA polymerase ...
Page 5-43
... Exonuclease Activity 5 ' CG 3 ' p 3 ' Gp Cp Ap Gp Cp Gp 5 ' DNA Polymerase Activity 5 ' 3 ' OH 3 ... Gp Cp Ap Gp Cp Gp 5 ' Bacteriophage T4 DNA Polymerase ( Bacteriophage T4 - infected E. Enzymes Used in Molecular Cloning 5.43.
... Exonuclease Activity 5 ' CG 3 ' p 3 ' Gp Cp Ap Gp Cp Gp 5 ' DNA Polymerase Activity 5 ' 3 ' OH 3 ... Gp Cp Ap Gp Cp Gp 5 ' Bacteriophage T4 DNA Polymerase ( Bacteriophage T4 - infected E. Enzymes Used in Molecular Cloning 5.43.
Page 5-73
... exonuclease that removes mononucleotides from both 3 ' termini of the two strands of linear DNA . BAL 31 is also an endonuclease ; thus , the single - stranded DNA generated by the 3 ' exonuclease activity is degraded by the endonuclease ...
... exonuclease that removes mononucleotides from both 3 ' termini of the two strands of linear DNA . BAL 31 is also an endonuclease ; thus , the single - stranded DNA generated by the 3 ' exonuclease activity is degraded by the endonuclease ...
Contents
BOOK | 1-2 |
DEVELOPMENT OF PLASMID CLONING VECTORS | 1-7 |
Index | 1-8 |
Copyright | |
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Common terms and phrases
agar agarose gel aliquots amber mutations bacteria bacteriophage M13 bacteriophage particles bacteriophage T4 bacteriophage T4 DNA BamHI Bgill buffer cDNA cells centrifugation at 12,000g cleaved cloning coli DNA polymerase colonies containing cosmid cosmid vector culture digestion DNA fragments DNA ligase DNA molecules DNA polymerase double-stranded DNA EcoRI EDTA efficiency Escherichia coli ethanol ethidium bromide eukaryotic eukaryotic DNA exonuclease extracts foreign DNA gel electrophoresis gene genetic Gp Cp HindIII Hindill host hybridization Incubate infected inserted intergenic region Kpnl lacZ linear lysogenic method methylase methylation microfuge tube minutes at 4°C nin5 nitrocellulose nucleotides origin of replication packaging pellet phagemids plaques plasmid plasmid DNA plate polycloning prepared protein Pvul recA recombinant red gam remove replication restriction enzyme room temperature Sacl Sall single-stranded DNA Smal solution sterile stored strains strand supernatant T4 DNA ligase teriophage Transfer Tris Cl pH vector DNA vitro volume Xbal µg/ml