Molecular Cloning: A Laboratory Manual, Volume 1 |
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Page 1-54
... Foreign DNA to Plasmid Vectors Termini carried by fragment of foreign DNA Blunt - ended Different protruding termini Identical protruding termini Requirements for cloning high concentrations of DNAs and ligase for maximum efficiency ...
... Foreign DNA to Plasmid Vectors Termini carried by fragment of foreign DNA Blunt - ended Different protruding termini Identical protruding termini Requirements for cloning high concentrations of DNAs and ligase for maximum efficiency ...
Page 4-33
... foreign DNA are inserted into bacteriophage M13 vectors in exactly the same way that they would be inserted into a ... DNA is ligated with an excess of foreign DNA that has compatible termini . No effort is made to reduce the background ...
... foreign DNA are inserted into bacteriophage M13 vectors in exactly the same way that they would be inserted into a ... DNA is ligated with an excess of foreign DNA that has compatible termini . No effort is made to reduce the background ...
Page 4-34
... DNA fragment . If the amount of foreign DNA is limited , use method 2 or 4 . • If the replicative form of the vector DNA has been cleaved with two different restriction enzymes , the fragment of foreign DNA can be inserted in only one ...
... DNA fragment . If the amount of foreign DNA is limited , use method 2 or 4 . • If the replicative form of the vector DNA has been cleaved with two different restriction enzymes , the fragment of foreign DNA can be inserted in only one ...
Contents
BOOK | 1-2 |
DEVELOPMENT OF PLASMID CLONING VECTORS | 1-7 |
Index | 1-8 |
Copyright | |
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Common terms and phrases
agar agarose gel aliquots amber mutations bacteria bacteriophage M13 bacteriophage particles bacteriophage T4 bacteriophage T4 DNA BamHI Bgill buffer cDNA cells centrifugation at 12,000g cleaved cloning coli DNA polymerase colonies containing cosmid cosmid vector culture digestion DNA fragments DNA ligase DNA molecules DNA polymerase double-stranded DNA EcoRI EDTA efficiency Escherichia coli ethanol ethidium bromide eukaryotic eukaryotic DNA exonuclease extracts foreign DNA gel electrophoresis gene genetic Gp Cp HindIII Hindill host hybridization Incubate infected inserted intergenic region Kpnl lacZ linear lysogenic method methylase methylation microfuge tube minutes at 4°C nin5 nitrocellulose nucleotides origin of replication packaging pellet phagemids plaques plasmid plasmid DNA plate polycloning prepared protein Pvul recA recombinant red gam remove replication restriction enzyme room temperature Sacl Sall single-stranded DNA Smal solution sterile stored strains strand supernatant T4 DNA ligase teriophage Transfer Tris Cl pH vector DNA vitro volume Xbal µg/ml