Molecular Cloning: A Laboratory Manual, Volume 1 |
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Page xxxiii
... Fusion Proteins 17.3 Vector Systems for the Expression of lacZ Fusion Genes 17.3 Construction of pUR - cDNA Plasmids and Detection of Fusion Proteins 17.7 Preparation of Fusion Proteins for Antibody Production Production of Intact Native ...
... Fusion Proteins 17.3 Vector Systems for the Expression of lacZ Fusion Genes 17.3 Construction of pUR - cDNA Plasmids and Detection of Fusion Proteins 17.7 Preparation of Fusion Proteins for Antibody Production Production of Intact Native ...
Page 2-43
... fusion protein in nonsuppressing E. coli . The temperature - sensitive repressor can be used to control bacteriophage replication and production of fusion protein . To construct Agt18 and Agt19 , the two bacteriophage A SalI sites were ...
... fusion protein in nonsuppressing E. coli . The temperature - sensitive repressor can be used to control bacteriophage replication and production of fusion protein . To construct Agt18 and Agt19 , the two bacteriophage A SalI sites were ...
Page 2-53
... fusion proteins , and production of capped RNA transcripts are all readily obtained . DNA fragments up to 10 kb in ... fusion protein may be expressed and detected by antibody screening . The Bluescript SK ( M13- ) plasmid carrying the ...
... fusion proteins , and production of capped RNA transcripts are all readily obtained . DNA fragments up to 10 kb in ... fusion protein may be expressed and detected by antibody screening . The Bluescript SK ( M13- ) plasmid carrying the ...
Contents
BOOK | 1-2 |
DEVELOPMENT OF PLASMID CLONING VECTORS | 1-7 |
Index | 1-8 |
Copyright | |
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Common terms and phrases
agar agarose gel aliquots amber mutations bacteria bacteriophage M13 bacteriophage particles bacteriophage T4 bacteriophage T4 DNA BamHI Bgill buffer cDNA cells centrifugation at 12,000g cleaved cloning coli DNA polymerase colonies containing cosmid cosmid vector culture digestion DNA fragments DNA ligase DNA molecules DNA polymerase double-stranded DNA EcoRI EDTA efficiency Escherichia coli ethanol ethidium bromide eukaryotic eukaryotic DNA exonuclease extracts foreign DNA gel electrophoresis gene genetic Gp Cp HindIII Hindill host hybridization Incubate infected inserted intergenic region Kpnl lacZ linear lysogenic method methylase methylation microfuge tube minutes at 4°C nin5 nitrocellulose nucleotides origin of replication packaging pellet phagemids plaques plasmid plasmid DNA plate polycloning prepared protein Pvul recA recombinant red gam remove replication restriction enzyme room temperature Sacl Sall single-stranded DNA Smal solution sterile stored strains strand supernatant T4 DNA ligase teriophage Transfer Tris Cl pH vector DNA vitro volume Xbal µg/ml