Molecular Cloning: A Laboratory Manual, Volume 1 |
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Page 1-108
... Genetic transforma- tion of Campylobacter jejuni with plas- mid DNA . Proc . Natl . Acad . Sci . 85 : 856 . Minton , N.P. , S.P. Chambers , S.E. Prior , S.T. Cole , and T. Garnier . 1988. Copy number and mobilization properties of PUC ...
... Genetic transforma- tion of Campylobacter jejuni with plas- mid DNA . Proc . Natl . Acad . Sci . 85 : 856 . Minton , N.P. , S.P. Chambers , S.E. Prior , S.T. Cole , and T. Garnier . 1988. Copy number and mobilization properties of PUC ...
Page 2-6
... genetic map of wild - type bacteriophage A. The general locations of genes that encode various lytic and lysogenic functions are indicated at the top of the figure by brackets . The genetic map below the brackets shows the specific ...
... genetic map of wild - type bacteriophage A. The general locations of genes that encode various lytic and lysogenic functions are indicated at the top of the figure by brackets . The genetic map below the brackets shows the specific ...
Page 4-3
... genetic map of bacteriophage M13 is shown in Figure 4.1 . Over 90 % of the viral genome codes for protein products , and most of the ten genes are separated by only a few nucleotides . The only extended intergenic regions lie between genes ...
... genetic map of bacteriophage M13 is shown in Figure 4.1 . Over 90 % of the viral genome codes for protein products , and most of the ten genes are separated by only a few nucleotides . The only extended intergenic regions lie between genes ...
Contents
BOOK | 1-2 |
DEVELOPMENT OF PLASMID CLONING VECTORS | 1-7 |
Index | 1-8 |
Copyright | |
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Common terms and phrases
agar agarose gel aliquots amber mutations bacteria bacteriophage M13 bacteriophage particles bacteriophage T4 bacteriophage T4 DNA BamHI Bgill buffer cDNA cells centrifugation at 12,000g cleaved cloning coli DNA polymerase colonies containing cosmid cosmid vector culture digestion DNA fragments DNA ligase DNA molecules DNA polymerase double-stranded DNA EcoRI EDTA efficiency Escherichia coli ethanol ethidium bromide eukaryotic eukaryotic DNA exonuclease extracts foreign DNA gel electrophoresis gene genetic Gp Cp HindIII Hindill host hybridization Incubate infected inserted intergenic region Kpnl lacZ linear lysogenic method methylase methylation microfuge tube minutes at 4°C nin5 nitrocellulose nucleotides origin of replication packaging pellet phagemids plaques plasmid plasmid DNA plate polycloning prepared protein Pvul recA recombinant red gam remove replication restriction enzyme room temperature Sacl Sall single-stranded DNA Smal solution sterile stored strains strand supernatant T4 DNA ligase teriophage Transfer Tris Cl pH vector DNA vitro volume Xbal µg/ml