Molecular Cloning: A Laboratory Manual, Volume 1 |
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Results 1-3 of 62
Page 2-70
... infected bacteria gives the best results ( Blattner et al . 1977 ; Maniatis et al . 1978 ) . 1. Place 100 ml of NZCYM in a 500 - ml flask and inoculate with a single colony of an appropriate bacterial host . Incubate overnight at 37 ° C ...
... infected bacteria gives the best results ( Blattner et al . 1977 ; Maniatis et al . 1978 ) . 1. Place 100 ml of NZCYM in a 500 - ml flask and inoculate with a single colony of an appropriate bacterial host . Incubate overnight at 37 ° C ...
Page 4-26
... infected bacteria by the same methods used to prepare plasmid DNA . Small - scale Preparations of the Replicative Form of Bacteriophage M13 DNA 1. Prepare an infected culture ( 2 ml ) as described on page 4.25 . To minimize the ...
... infected bacteria by the same methods used to prepare plasmid DNA . Small - scale Preparations of the Replicative Form of Bacteriophage M13 DNA 1. Prepare an infected culture ( 2 ml ) as described on page 4.25 . To minimize the ...
Page 4-31
... infected cells into 250 ml of fresh LB medium , prewarmed to 37 ° C , in a 2 - liter flask . Incubate for 5 hours at 37 ° C with constant , vigorous shaking . 4. Harvest the infected cells by centrifugation at 4000g for 15 minutes at 4 ...
... infected cells into 250 ml of fresh LB medium , prewarmed to 37 ° C , in a 2 - liter flask . Incubate for 5 hours at 37 ° C with constant , vigorous shaking . 4. Harvest the infected cells by centrifugation at 4000g for 15 minutes at 4 ...
Contents
BOOK | 1-2 |
DEVELOPMENT OF PLASMID CLONING VECTORS | 1-7 |
Index | 1-8 |
Copyright | |
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Common terms and phrases
agar agarose gel aliquots amber mutations bacteria bacteriophage M13 bacteriophage particles bacteriophage T4 bacteriophage T4 DNA BamHI Bgill buffer cDNA cells centrifugation at 12,000g cleaved cloning coli DNA polymerase colonies containing cosmid cosmid vector culture digestion DNA fragments DNA ligase DNA molecules DNA polymerase double-stranded DNA EcoRI EDTA efficiency Escherichia coli ethanol ethidium bromide eukaryotic eukaryotic DNA exonuclease extracts foreign DNA gel electrophoresis gene genetic Gp Cp HindIII Hindill host hybridization Incubate infected inserted intergenic region Kpnl lacZ linear lysogenic method methylase methylation microfuge tube minutes at 4°C nin5 nitrocellulose nucleotides origin of replication packaging pellet phagemids plaques plasmid plasmid DNA plate polycloning prepared protein Pvul recA recombinant red gam remove replication restriction enzyme room temperature Sacl Sall single-stranded DNA Smal solution sterile stored strains strand supernatant T4 DNA ligase teriophage Transfer Tris Cl pH vector DNA vitro volume Xbal µg/ml