Molecular Cloning: A Laboratory Manual, Volume 1 |
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Results 1-3 of 75
Page 1-54
... inserted in only one orientation within recombinant plasmid restriction sites at junctions between plasmid and foreign DNAs are usually conserved foreign DNA can be inserted in either orientation . recombinant plasmids may carry tandem ...
... inserted in only one orientation within recombinant plasmid restriction sites at junctions between plasmid and foreign DNAs are usually conserved foreign DNA can be inserted in either orientation . recombinant plasmids may carry tandem ...
Page 1-87
... insertion of a segment of rat pre- proinsulin cDNA into the PstI site of pBR322 did not inactivate the amp gene . Presumably , a small piece of foreign DNA had been inserted that did not alter the reading frame of the amp ' gene , so ...
... insertion of a segment of rat pre- proinsulin cDNA into the PstI site of pBR322 did not inactivate the amp gene . Presumably , a small piece of foreign DNA had been inserted that did not alter the reading frame of the amp ' gene , so ...
Page 4-33
... inserted into bacteriophage M13 vectors in exactly the same way that they would be inserted into a conventional plasmid . Four different methods are commonly used : 1. The polycloning site of the vector is cleaved with a single ...
... inserted into bacteriophage M13 vectors in exactly the same way that they would be inserted into a conventional plasmid . Four different methods are commonly used : 1. The polycloning site of the vector is cleaved with a single ...
Contents
BOOK | 1-2 |
DEVELOPMENT OF PLASMID CLONING VECTORS | 1-7 |
Index | 1-8 |
Copyright | |
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agar agarose gel aliquots amber mutations bacteria bacteriophage M13 bacteriophage particles bacteriophage T4 bacteriophage T4 DNA BamHI Bgill buffer cDNA cells centrifugation at 12,000g cleaved cloning coli DNA polymerase colonies containing cosmid cosmid vector culture digestion DNA fragments DNA ligase DNA molecules DNA polymerase double-stranded DNA EcoRI EDTA efficiency Escherichia coli ethanol ethidium bromide eukaryotic eukaryotic DNA exonuclease extracts foreign DNA gel electrophoresis gene genetic Gp Cp HindIII Hindill host hybridization Incubate infected inserted intergenic region Kpnl lacZ linear lysogenic method methylase methylation microfuge tube minutes at 4°C nin5 nitrocellulose nucleotides origin of replication packaging pellet phagemids plaques plasmid plasmid DNA plate polycloning prepared protein Pvul recA recombinant red gam remove replication restriction enzyme room temperature Sacl Sall single-stranded DNA Smal solution sterile stored strains strand supernatant T4 DNA ligase teriophage Transfer Tris Cl pH vector DNA vitro volume Xbal µg/ml