Molecular Cloning: A Laboratory Manual, Book 1 |
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User Review - iayork - LibraryThingthe BIBLE of every biologist: So few and so much to say about this bible of Biology at the bench... You'll really find everything you want in it, including the composition of all the buffers and ... Read full review
LibraryThing Review
User Review - iayork - LibraryThingthe BIBLE of every biologist: So few and so much to say about this bible of Biology at the bench... You'll really find everything you want in it, including the composition of all the buffers and ... Read full review
Contents
Mobilization 1 | 1-5 |
Constructing Expression Libraries in Plasmid and Bacteriophage | 1-12 |
Oligonucleotidemediated Mutagenesis 15 51 | 1-15 |
Copyright | |
86 other sections not shown
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Common terms and phrases
acrylamide agar agarose gel aliquots amber mutations bacterial bacteriophage M13 bacteriophage particles bacteriophage T4 DNA Bgill Bgll Bøml buffer carrying CCWGG cDNA cells centrifugation Cl pH cleavage cleaved cloning cohesive termini coli DNA polymerase colonies containing cosmid cosmid vector culture digestion DNA fragments DNA ligase DNA molecules double-stranded DNA EcoRI EDTA efficiency Escherichia coli ethanol ethidium bromide eukaryotic eukaryotic DNA exonuclease exonuclease activity extracts foreign DNA GATC gel electrophoresis gene genetic Gp Cp HindIII host hybridization Incubate infected inserted isoschizomers linear lysogenic method methylase methylation minutes at 4°C nicks nitrocellulose nitrocellulose filters nuclease Nucleic Acids nucleotides packaging pellet phagemids plaques plasmid plasmid DNA plate polycloning prepared probes protein Pvul recA recombinant red gam remove replication restriction enzyme RNAase room temperature SacI sequences single-stranded DNA solution stored strains strand Substrate supernatant T4 DNA ligase template teriophage ug/ml vector DNA vitro volume Xbal
Bibliographic information
| Title | Molecular Cloning: A Laboratory Manual, Book 1 Molecular Cloning: A Laboratory Manual, Joseph Sambrook, ISBN 0879693096, 9780879693091 |
| Authors | Joseph Sambrook, Tom Maniatis |
| Photographs by | E. F. Fritsch |
| Edition | 2 |
| Publisher | Cold Spring Harbor Laboratory, 1989 |
| Original from | the University of Michigan |
| Digitized | 23 Feb 2010 |
| ISBN | 0879693096, 9780879693091 |
| Length | 3 pages |
| Export Citation | BiBTeX EndNote RefMan |