Molecular Cloning: A Laboratory Manual, Volume 1 |
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Results 1-3 of 77
Page 6-6
... length of the gel itself . DIRECTION OF THE ELECTRIC FIELD DNA molecules larger than 50-100 kb in length migrate through agarose gels at the same rate if the direction of the electric field remains constant . However , if the direction ...
... length of the gel itself . DIRECTION OF THE ELECTRIC FIELD DNA molecules larger than 50-100 kb in length migrate through agarose gels at the same rate if the direction of the electric field remains constant . However , if the direction ...
Page 6-50
... length . The importance of this limitation becomes apparent when it is realized that a single genetic locus ( e.g. , the major histocompatibility locus of mammals ) may occupy several thousand kilobases of DNA and that DNA molecules in ...
... length . The importance of this limitation becomes apparent when it is realized that a single genetic locus ( e.g. , the major histocompatibility locus of mammals ) may occupy several thousand kilobases of DNA and that DNA molecules in ...
Page 7-77
... length by agarose or polyacrylamide gel electrophoresis . iii . Problems occasionally arise because of degradation of the probe before the hybridization reaction or , more rarely , because of the synthesis of tran- scripts that are less ...
... length by agarose or polyacrylamide gel electrophoresis . iii . Problems occasionally arise because of degradation of the probe before the hybridization reaction or , more rarely , because of the synthesis of tran- scripts that are less ...
Contents
BOOK | 1-2 |
DEVELOPMENT OF PLASMID CLONING VECTORS | 1-7 |
Index | 1-8 |
Copyright | |
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Common terms and phrases
agar agarose gel aliquots amber mutations bacteria bacteriophage M13 bacteriophage particles bacteriophage T4 bacteriophage T4 DNA BamHI Bgill buffer cDNA cells centrifugation at 12,000g cleaved cloning coli DNA polymerase colonies containing cosmid cosmid vector culture digestion DNA fragments DNA ligase DNA molecules DNA polymerase double-stranded DNA EcoRI EDTA efficiency Escherichia coli ethanol ethidium bromide eukaryotic eukaryotic DNA exonuclease extracts foreign DNA gel electrophoresis gene genetic Gp Cp HindIII Hindill host hybridization Incubate infected inserted intergenic region Kpnl lacZ linear lysogenic method methylase methylation microfuge tube minutes at 4°C nin5 nitrocellulose nucleotides origin of replication packaging pellet phagemids plaques plasmid plasmid DNA plate polycloning prepared protein Pvul recA recombinant red gam remove replication restriction enzyme room temperature Sacl Sall single-stranded DNA Smal solution sterile stored strains strand supernatant T4 DNA ligase teriophage Transfer Tris Cl pH vector DNA vitro volume Xbal µg/ml