Molecular Cloning: A Laboratory Manual, Volume 1 |
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Page 2-8
... lysogenic pathways depends on an intricate balance between the activities of a number of host and bacteriophage gene products in the infected cell . To establish lysogeny , proteins encoded by genes cII and cIII ( whose transcriptions ...
... lysogenic pathways depends on an intricate balance between the activities of a number of host and bacteriophage gene products in the infected cell . To establish lysogeny , proteins encoded by genes cII and cIII ( whose transcriptions ...
Page 2-95
... lysogens of the appropriate genotype ( amber mutations in the A , D , or E gene ) . The lysogens also carry one or more of ... lysogenic state when the host bacteria are grown at 32 ° C ; lytic growth of the bacteriophage is induced by ...
... lysogens of the appropriate genotype ( amber mutations in the A , D , or E gene ) . The lysogens also carry one or more of ... lysogenic state when the host bacteria are grown at 32 ° C ; lytic growth of the bacteriophage is induced by ...
Page 2-98
... lysogenic strain is used , and only one extract is prepared . ( 2 ) The lysogenic bacteria are a strain of E. coli C. A single lysogen can be used to prepare packaging extracts for two reasons . First , the bacteriophage A genome lysogenic ...
... lysogenic strain is used , and only one extract is prepared . ( 2 ) The lysogenic bacteria are a strain of E. coli C. A single lysogen can be used to prepare packaging extracts for two reasons . First , the bacteriophage A genome lysogenic ...
Contents
BOOK | 1-2 |
DEVELOPMENT OF PLASMID CLONING VECTORS | 1-7 |
Index | 1-8 |
Copyright | |
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Common terms and phrases
agar agarose gel aliquots amber mutations bacteria bacteriophage M13 bacteriophage particles bacteriophage T4 bacteriophage T4 DNA BamHI Bgill buffer cDNA cells centrifugation at 12,000g cleaved cloning coli DNA polymerase colonies containing cosmid cosmid vector culture digestion DNA fragments DNA ligase DNA molecules DNA polymerase double-stranded DNA EcoRI EDTA efficiency Escherichia coli ethanol ethidium bromide eukaryotic eukaryotic DNA exonuclease extracts foreign DNA gel electrophoresis gene genetic Gp Cp HindIII Hindill host hybridization Incubate infected inserted intergenic region Kpnl lacZ linear lysogenic method methylase methylation microfuge tube minutes at 4°C nin5 nitrocellulose nucleotides origin of replication packaging pellet phagemids plaques plasmid plasmid DNA plate polycloning prepared protein Pvul recA recombinant red gam remove replication restriction enzyme room temperature Sacl Sall single-stranded DNA Smal solution sterile stored strains strand supernatant T4 DNA ligase teriophage Transfer Tris Cl pH vector DNA vitro volume Xbal µg/ml