Molecular Cloning: A Laboratory Manual, Volume 1 |
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Page xxii
... mRNA 8.3 Integrity of the mRNA 8.4 Abundant mRNAs 8.6 Low - abundance mRNAs 8.6 Methods of Enrichment 8.6 FRACTIONATION OF mRNA BY SIZE 8.8 FRACTIONATION OF cDNA 8.8 IMMUNOLOGICAL PURIFICATION OF POLYSOMES 8.9 SYNTHESIS OF THE FIRST ...
... mRNA 8.3 Integrity of the mRNA 8.4 Abundant mRNAs 8.6 Low - abundance mRNAs 8.6 Methods of Enrichment 8.6 FRACTIONATION OF mRNA BY SIZE 8.8 FRACTIONATION OF cDNA 8.8 IMMUNOLOGICAL PURIFICATION OF POLYSOMES 8.9 SYNTHESIS OF THE FIRST ...
Page 7-37
... mRNA molecules in preparations of cellular RNA . These include : • Northern hybridization ( RNA blotting ) , in which the size and amount of specific mRNA molecules in preparations of total or poly ( A ) * RNA are determined ( Alwine et ...
... mRNA molecules in preparations of cellular RNA . These include : • Northern hybridization ( RNA blotting ) , in which the size and amount of specific mRNA molecules in preparations of total or poly ( A ) * RNA are determined ( Alwine et ...
Page 7-71
... mRNA ( Zinn et al . 1983 ) . The strategy for mapping mRNA with radiolabeled RNA probes generated in vitro is illustrated in Figure 7.6 . A segment of DNA containing all or part of the gene of interest is inserted into a polycloning ...
... mRNA ( Zinn et al . 1983 ) . The strategy for mapping mRNA with radiolabeled RNA probes generated in vitro is illustrated in Figure 7.6 . A segment of DNA containing all or part of the gene of interest is inserted into a polycloning ...
Contents
BOOK | 1-2 |
DEVELOPMENT OF PLASMID CLONING VECTORS | 1-7 |
Index | 1-8 |
Copyright | |
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Common terms and phrases
agar agarose gel aliquots amber mutations bacteria bacteriophage M13 bacteriophage particles bacteriophage T4 bacteriophage T4 DNA BamHI Bgill buffer cDNA cells centrifugation at 12,000g cleaved cloning coli DNA polymerase colonies containing cosmid cosmid vector culture digestion DNA fragments DNA ligase DNA molecules DNA polymerase double-stranded DNA EcoRI EDTA efficiency Escherichia coli ethanol ethidium bromide eukaryotic eukaryotic DNA exonuclease extracts foreign DNA gel electrophoresis gene genetic Gp Cp HindIII Hindill host hybridization Incubate infected inserted intergenic region Kpnl lacZ linear lysogenic method methylase methylation microfuge tube minutes at 4°C nin5 nitrocellulose nucleotides origin of replication packaging pellet phagemids plaques plasmid plasmid DNA plate polycloning prepared protein Pvul recA recombinant red gam remove replication restriction enzyme room temperature Sacl Sall single-stranded DNA Smal solution sterile stored strains strand supernatant T4 DNA ligase teriophage Transfer Tris Cl pH vector DNA vitro volume Xbal µg/ml