Molecular Cloning: A Laboratory Manual, Volume 1 |
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Page xxxii
... Mammalian Cells Expression of Proteins 16.3 EXPRESSION OF PROTEINS FROM CLONED GENES 16.3 FUNCTIONAL COMPONENTS OF MAMMALIAN EXPRESSION VECTORS 16.5 Prokaryotic Plasmid Sequences That Facilitate the Construction , Propagation , and ...
... Mammalian Cells Expression of Proteins 16.3 EXPRESSION OF PROTEINS FROM CLONED GENES 16.3 FUNCTIONAL COMPONENTS OF MAMMALIAN EXPRESSION VECTORS 16.5 Prokaryotic Plasmid Sequences That Facilitate the Construction , Propagation , and ...
Page 3-18
... MAMMALIAN CELLS Several cosmid vectors have been developed to shuttle genes into and out of mammalian cells and to isolate eukaryotic genes by functional selection . These vectors differ from one another chiefly in the prokaryotic and ...
... MAMMALIAN CELLS Several cosmid vectors have been developed to shuttle genes into and out of mammalian cells and to isolate eukaryotic genes by functional selection . These vectors differ from one another chiefly in the prokaryotic and ...
Page 5-26
... mammalian chromosomal DNA recognize sequences that contain CpG . Because this dinucleotide occurs approximately fivefold less frequently in mammalian DNA than expected ( Normore 1976 ; Setlow 1976 ; Shapiro 1976 ) , restriction enzyme ...
... mammalian chromosomal DNA recognize sequences that contain CpG . Because this dinucleotide occurs approximately fivefold less frequently in mammalian DNA than expected ( Normore 1976 ; Setlow 1976 ; Shapiro 1976 ) , restriction enzyme ...
Contents
BOOK | 1-2 |
DEVELOPMENT OF PLASMID CLONING VECTORS | 1-7 |
Index | 1-8 |
Copyright | |
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agar agarose gel aliquots amber mutations bacteria bacteriophage M13 bacteriophage particles bacteriophage T4 bacteriophage T4 DNA BamHI Bgill buffer cDNA cells centrifugation at 12,000g cleaved cloning coli DNA polymerase colonies containing cosmid cosmid vector culture digestion DNA fragments DNA ligase DNA molecules DNA polymerase double-stranded DNA EcoRI EDTA efficiency Escherichia coli ethanol ethidium bromide eukaryotic eukaryotic DNA exonuclease extracts foreign DNA gel electrophoresis gene genetic Gp Cp HindIII Hindill host hybridization Incubate infected inserted intergenic region Kpnl lacZ linear lysogenic method methylase methylation microfuge tube minutes at 4°C nin5 nitrocellulose nucleotides origin of replication packaging pellet phagemids plaques plasmid plasmid DNA plate polycloning prepared protein Pvul recA recombinant red gam remove replication restriction enzyme room temperature Sacl Sall single-stranded DNA Smal solution sterile stored strains strand supernatant T4 DNA ligase teriophage Transfer Tris Cl pH vector DNA vitro volume Xbal µg/ml