Molecular Cloning: A Laboratory Manual, Volume 1 |
From inside the book
Results 1-3 of 82
Page 1-23
... method can therefore be used with any strain of E. coli grown in cultures of any size . It is curious , however , that many laboratories use one method to process minipreps and another method to isolate plasmid DNA from large - scale ...
... method can therefore be used with any strain of E. coli grown in cultures of any size . It is curious , however , that many laboratories use one method to process minipreps and another method to isolate plasmid DNA from large - scale ...
Page 2-118
... method ( E.F. Fritsch , unpubl . ) and the liquid culture method ( Leder et al . 1977 ) . Both methods work equally well , although there are occasional problems with the yield and purity of the bacteriophage DNA . If trouble should ...
... method ( E.F. Fritsch , unpubl . ) and the liquid culture method ( Leder et al . 1977 ) . Both methods work equally well , although there are occasional problems with the yield and purity of the bacteriophage DNA . If trouble should ...
Page 6-17
... Method 1 The following method is from Lunn and Sansone ( 1987 ) . 1. Add 2.9 g of Amberlite XAD - 16 for each 100 ml of solution . Amberlite XAD - 16 , a nonionic , polymeric absorbent , is available from Rohm and Haas . 2. Store the ...
... Method 1 The following method is from Lunn and Sansone ( 1987 ) . 1. Add 2.9 g of Amberlite XAD - 16 for each 100 ml of solution . Amberlite XAD - 16 , a nonionic , polymeric absorbent , is available from Rohm and Haas . 2. Store the ...
Contents
BOOK | 1-2 |
DEVELOPMENT OF PLASMID CLONING VECTORS | 1-7 |
Index | 1-8 |
Copyright | |
84 other sections not shown
Other editions - View all
Common terms and phrases
agar agarose gel aliquots amber mutations bacteria bacteriophage M13 bacteriophage particles bacteriophage T4 bacteriophage T4 DNA BamHI Bgill buffer cDNA cells centrifugation at 12,000g cleaved cloning coli DNA polymerase colonies containing cosmid cosmid vector culture digestion DNA fragments DNA ligase DNA molecules DNA polymerase double-stranded DNA EcoRI EDTA efficiency Escherichia coli ethanol ethidium bromide eukaryotic eukaryotic DNA exonuclease extracts foreign DNA gel electrophoresis gene genetic Gp Cp HindIII Hindill host hybridization Incubate infected inserted intergenic region Kpnl lacZ linear lysogenic method methylase methylation microfuge tube minutes at 4°C nin5 nitrocellulose nucleotides origin of replication packaging pellet phagemids plaques plasmid plasmid DNA plate polycloning prepared protein Pvul recA recombinant red gam remove replication restriction enzyme room temperature Sacl Sall single-stranded DNA Smal solution sterile stored strains strand supernatant T4 DNA ligase teriophage Transfer Tris Cl pH vector DNA vitro volume Xbal µg/ml