Molecular Cloning: A Laboratory Manual, Volume 1 |
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Page xviii
... METHYLASE 5.15 dem METHYLASE 5.15 METHYLATION - DEPENDENT RESTRICTION SYSTEMS IN E. coli M.EcoRI METHYLASE 5.15 Modification of Restriction Sites by DNA Methylation 5.16 Influence of Methylation on DNA Mapping 5.26 ~ DIGESTING DNA WITH ...
... METHYLASE 5.15 dem METHYLASE 5.15 METHYLATION - DEPENDENT RESTRICTION SYSTEMS IN E. coli M.EcoRI METHYLASE 5.15 Modification of Restriction Sites by DNA Methylation 5.16 Influence of Methylation on DNA Mapping 5.26 ~ DIGESTING DNA WITH ...
Page 2-57
... methylase . hsdS strains are defective in both restriction and methylation ( for a review , see Yuan 1981 ) . Bacterial gene encoding the subunit of E. coli methylase that methylates EcoK restriction sites . The EcoK restriction enzyme ...
... methylase . hsdS strains are defective in both restriction and methylation ( for a review , see Yuan 1981 ) . Bacterial gene encoding the subunit of E. coli methylase that methylates EcoK restriction sites . The EcoK restriction enzyme ...
Page 5-15
... methylase and the dcm methylase . dam METHYLASE This enzyme introduces methyl groups at the N ° position of adenine in the sequence 5 ′ GATC 3 ' ( Hattman et al . 1978 ) . The recognition sites of several restriction enzymes ( PvuI ...
... methylase and the dcm methylase . dam METHYLASE This enzyme introduces methyl groups at the N ° position of adenine in the sequence 5 ′ GATC 3 ' ( Hattman et al . 1978 ) . The recognition sites of several restriction enzymes ( PvuI ...
Contents
BOOK | 1-2 |
DEVELOPMENT OF PLASMID CLONING VECTORS | 1-7 |
Index | 1-8 |
Copyright | |
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agar agarose gel aliquots amber mutations bacteria bacteriophage M13 bacteriophage particles bacteriophage T4 bacteriophage T4 DNA BamHI Bgill buffer cDNA cells centrifugation at 12,000g cleaved cloning coli DNA polymerase colonies containing cosmid cosmid vector culture digestion DNA fragments DNA ligase DNA molecules DNA polymerase double-stranded DNA EcoRI EDTA efficiency Escherichia coli ethanol ethidium bromide eukaryotic eukaryotic DNA exonuclease extracts foreign DNA gel electrophoresis gene genetic Gp Cp HindIII Hindill host hybridization Incubate infected inserted intergenic region Kpnl lacZ linear lysogenic method methylase methylation microfuge tube minutes at 4°C nin5 nitrocellulose nucleotides origin of replication packaging pellet phagemids plaques plasmid plasmid DNA plate polycloning prepared protein Pvul recA recombinant red gam remove replication restriction enzyme room temperature Sacl Sall single-stranded DNA Smal solution sterile stored strains strand supernatant T4 DNA ligase teriophage Transfer Tris Cl pH vector DNA vitro volume Xbal µg/ml