Molecular Cloning: A Laboratory Manual, Volume 1 |
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Page 5-15
A Laboratory Manual Joseph Sambrook, E. F. Fritsch, Tom Maniatis. DNA METHYLATION Methylation by Commonly Used ... methylation . ( Note : Mammalian DNA is not methylated at the N ° position of adenine , and therefore either Mbol or Sau3AI ...
A Laboratory Manual Joseph Sambrook, E. F. Fritsch, Tom Maniatis. DNA METHYLATION Methylation by Commonly Used ... methylation . ( Note : Mammalian DNA is not methylated at the N ° position of adenine , and therefore either Mbol or Sau3AI ...
Page 5-25
... Methylation Methylated sequenceb Tm5 CCGGA TCmsCGGA GGWC5C TCCGGm6A Cm5 CWGG GGTACT5C Cm5 CGG Cm4CGG Gm6ATC TCGCGm6A ... methylation if it cuts the methylated sequence at a rate that is at least one tenth the rate at which it cuts the ...
... Methylation Methylated sequenceb Tm5 CCGGA TCmsCGGA GGWC5C TCCGGm6A Cm5 CWGG GGTACT5C Cm5 CGG Cm4CGG Gm6ATC TCGCGm6A ... methylation if it cuts the methylated sequence at a rate that is at least one tenth the rate at which it cuts the ...
Page 5-26
... methylation is highly cell - type specific ( Bird and Southern 1978 ) ; any given CpG doublet is methylated in the ... methylation . Finally , methylation of CpG dinucleotides in prepara- tions of mammalian DNA is rarely complete . This ...
... methylation is highly cell - type specific ( Bird and Southern 1978 ) ; any given CpG doublet is methylated in the ... methylation . Finally , methylation of CpG dinucleotides in prepara- tions of mammalian DNA is rarely complete . This ...
Contents
BOOK | 1-2 |
DEVELOPMENT OF PLASMID CLONING VECTORS | 1-7 |
Index | 1-8 |
Copyright | |
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agar agarose gel aliquots amber mutations bacteria bacteriophage M13 bacteriophage particles bacteriophage T4 bacteriophage T4 DNA BamHI Bgill buffer cDNA cells centrifugation at 12,000g cleaved cloning coli DNA polymerase colonies containing cosmid cosmid vector culture digestion DNA fragments DNA ligase DNA molecules DNA polymerase double-stranded DNA EcoRI EDTA efficiency Escherichia coli ethanol ethidium bromide eukaryotic eukaryotic DNA exonuclease extracts foreign DNA gel electrophoresis gene genetic Gp Cp HindIII Hindill host hybridization Incubate infected inserted intergenic region Kpnl lacZ linear lysogenic method methylase methylation microfuge tube minutes at 4°C nin5 nitrocellulose nucleotides origin of replication packaging pellet phagemids plaques plasmid plasmid DNA plate polycloning prepared protein Pvul recA recombinant red gam remove replication restriction enzyme room temperature Sacl Sall single-stranded DNA Smal solution sterile stored strains strand supernatant T4 DNA ligase teriophage Transfer Tris Cl pH vector DNA vitro volume Xbal µg/ml