Molecular Cloning: A Laboratory Manual, Volume 1 |
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Page 1-29
... tube in a boiling - water bath for exactly 40 seconds . 4. Centrifuge the bacterial lysate at 12,000g for 10 minutes at room temperature in a microfuge . 5. Remove the pellet of bacterial debris from the microfuge tube with a sterile ...
... tube in a boiling - water bath for exactly 40 seconds . 4. Centrifuge the bacterial lysate at 12,000g for 10 minutes at room temperature in a microfuge . 5. Remove the pellet of bacterial debris from the microfuge tube with a sterile ...
Page 1-40
... tube , and add 3 ml of an ice - cold solution of 5 м LiCl . Mix well , and then centrifuge the solution at 10,000 ... microfuge tube and store at room temperature for 30 minutes . 5. Add 500 μl of 1.6 M NaCl containing 13 % ( w / v ) ...
... tube , and add 3 ml of an ice - cold solution of 5 м LiCl . Mix well , and then centrifuge the solution at 10,000 ... microfuge tube and store at room temperature for 30 minutes . 5. Add 500 μl of 1.6 M NaCl containing 13 % ( w / v ) ...
Page 6-26
... microfuge tube . Add enough high - salt elution buffer to cover the membrane completely . The membrane should be crushed or folded gently , but not tightly packed . Cap the tube and incubate for 30 minutes at 65 ° C . Check the tube ...
... microfuge tube . Add enough high - salt elution buffer to cover the membrane completely . The membrane should be crushed or folded gently , but not tightly packed . Cap the tube and incubate for 30 minutes at 65 ° C . Check the tube ...
Contents
BOOK | 1-2 |
DEVELOPMENT OF PLASMID CLONING VECTORS | 1-7 |
Index | 1-8 |
Copyright | |
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Common terms and phrases
agar agarose gel aliquots amber mutations bacteria bacteriophage M13 bacteriophage particles bacteriophage T4 bacteriophage T4 DNA BamHI Bgill buffer cDNA cells centrifugation at 12,000g cleaved cloning coli DNA polymerase colonies containing cosmid cosmid vector culture digestion DNA fragments DNA ligase DNA molecules DNA polymerase double-stranded DNA EcoRI EDTA efficiency Escherichia coli ethanol ethidium bromide eukaryotic eukaryotic DNA exonuclease extracts foreign DNA gel electrophoresis gene genetic Gp Cp HindIII Hindill host hybridization Incubate infected inserted intergenic region Kpnl lacZ linear lysogenic method methylase methylation microfuge tube minutes at 4°C nin5 nitrocellulose nucleotides origin of replication packaging pellet phagemids plaques plasmid plasmid DNA plate polycloning prepared protein Pvul recA recombinant red gam remove replication restriction enzyme room temperature Sacl Sall single-stranded DNA Smal solution sterile stored strains strand supernatant T4 DNA ligase teriophage Transfer Tris Cl pH vector DNA vitro volume Xbal µg/ml