Molecular Cloning: A Laboratory Manual, Volume 1 |
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Page xxx
... Mutagenesis of Cloned DNA Generation of Deletions and Insertions 15.3 SIMPLE DELETIONS OR INSERTIONS 15.3 SYSTEMATIC DELETIONS AND INSERTIONS 15.5 Linker - insertion Mutagenesis 15.5 GENERATION OF LINKER - INSERTION MUTANTS WITH ...
... Mutagenesis of Cloned DNA Generation of Deletions and Insertions 15.3 SIMPLE DELETIONS OR INSERTIONS 15.3 SYSTEMATIC DELETIONS AND INSERTIONS 15.5 Linker - insertion Mutagenesis 15.5 GENERATION OF LINKER - INSERTION MUTANTS WITH ...
Page xxxi
... Mutagenesis 15.61 OLIGONUCLEOTIDE - MEDIATED MUTAGENESIS BY THE DOUBLE PRIMER METHOD 15.63 SCREENING BACTERIOPHAGE M13 PLAQUES AND COLONIES BY HYBRIDIZATION TO RADIOLABELED OLIGONUCLEOTIDES 15.66 Radiolabeling of Oligonucleotides by ...
... Mutagenesis 15.61 OLIGONUCLEOTIDE - MEDIATED MUTAGENESIS BY THE DOUBLE PRIMER METHOD 15.63 SCREENING BACTERIOPHAGE M13 PLAQUES AND COLONIES BY HYBRIDIZATION TO RADIOLABELED OLIGONUCLEOTIDES 15.66 Radiolabeling of Oligonucleotides by ...
Page 4-34
... mutagenesis . However , directional cloning may impose limitations when the single - stranded templates are to be used to determine the sequence of the foreign DNA or to generate strand - specific probes . If " universal primer " is ...
... mutagenesis . However , directional cloning may impose limitations when the single - stranded templates are to be used to determine the sequence of the foreign DNA or to generate strand - specific probes . If " universal primer " is ...
Contents
BOOK | 1-2 |
DEVELOPMENT OF PLASMID CLONING VECTORS | 1-7 |
Index | 1-8 |
Copyright | |
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Common terms and phrases
agar agarose gel aliquots amber mutations bacteria bacteriophage M13 bacteriophage particles bacteriophage T4 bacteriophage T4 DNA BamHI Bgill buffer cDNA cells centrifugation at 12,000g cleaved cloning coli DNA polymerase colonies containing cosmid cosmid vector culture digestion DNA fragments DNA ligase DNA molecules DNA polymerase double-stranded DNA EcoRI EDTA efficiency Escherichia coli ethanol ethidium bromide eukaryotic eukaryotic DNA exonuclease extracts foreign DNA gel electrophoresis gene genetic Gp Cp HindIII Hindill host hybridization Incubate infected inserted intergenic region Kpnl lacZ linear lysogenic method methylase methylation microfuge tube minutes at 4°C nin5 nitrocellulose nucleotides origin of replication packaging pellet phagemids plaques plasmid plasmid DNA plate polycloning prepared protein Pvul recA recombinant red gam remove replication restriction enzyme room temperature Sacl Sall single-stranded DNA Smal solution sterile stored strains strand supernatant T4 DNA ligase teriophage Transfer Tris Cl pH vector DNA vitro volume Xbal µg/ml