Molecular Cloning: A Laboratory Manual, Volume 1 |
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Page 2-59
... mutations Used to screen recombinants made in vectors carrying a lacZ gene Nonpermissive for vectors carrying amber mutations Will not modify or restrict DNA recA strain Permissive for vectors carrying amber mutations Will not modify or ...
... mutations Used to screen recombinants made in vectors carrying a lacZ gene Nonpermissive for vectors carrying amber mutations Will not modify or restrict DNA recA strain Permissive for vectors carrying amber mutations Will not modify or ...
Page 2-95
... mutations in the A , D , or E gene ) . The lysogens also carry one or more of the following mutations : • cIts857 or imm434 clts , which specify a temperature - sensitive bacterio- phage A repressor molecule . This mutation causes ...
... mutations in the A , D , or E gene ) . The lysogens also carry one or more of the following mutations : • cIts857 or imm434 clts , which specify a temperature - sensitive bacterio- phage A repressor molecule . This mutation causes ...
Page 4-14
... mutations Messing ( 1979 ) rk F ' traD36 proAB * lacl lacZAM15 supE △ lac - proAB ) hsdR4 JM105 rk rk Rec F ... mutations Yanisch - Perron et al . ( 1985 ) Will modify but not restrict transfected DNA Yanisch - Perron et al . ( 1985 ) ...
... mutations Messing ( 1979 ) rk F ' traD36 proAB * lacl lacZAM15 supE △ lac - proAB ) hsdR4 JM105 rk rk Rec F ... mutations Yanisch - Perron et al . ( 1985 ) Will modify but not restrict transfected DNA Yanisch - Perron et al . ( 1985 ) ...
Contents
BOOK | 1-2 |
DEVELOPMENT OF PLASMID CLONING VECTORS | 1-7 |
Index | 1-8 |
Copyright | |
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Common terms and phrases
agar agarose gel aliquots amber mutations bacteria bacteriophage M13 bacteriophage particles bacteriophage T4 bacteriophage T4 DNA BamHI Bgill buffer cDNA cells centrifugation at 12,000g cleaved cloning coli DNA polymerase colonies containing cosmid cosmid vector culture digestion DNA fragments DNA ligase DNA molecules DNA polymerase double-stranded DNA EcoRI EDTA efficiency Escherichia coli ethanol ethidium bromide eukaryotic eukaryotic DNA exonuclease extracts foreign DNA gel electrophoresis gene genetic Gp Cp HindIII Hindill host hybridization Incubate infected inserted intergenic region Kpnl lacZ linear lysogenic method methylase methylation microfuge tube minutes at 4°C nin5 nitrocellulose nucleotides origin of replication packaging pellet phagemids plaques plasmid plasmid DNA plate polycloning prepared protein Pvul recA recombinant red gam remove replication restriction enzyme room temperature Sacl Sall single-stranded DNA Smal solution sterile stored strains strand supernatant T4 DNA ligase teriophage Transfer Tris Cl pH vector DNA vitro volume Xbal µg/ml