Molecular Cloning: A Laboratory Manual, Volume 1 |
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Page 1-92
... filter laid on the surface of a second agar plate . After a period of growth , the colonies are lysed in situ . The master plate is stored at 4 ° C until the results of the screening procedure become available ... Nitrocellulose Filters 1.
... filter laid on the surface of a second agar plate . After a period of growth , the colonies are lysed in situ . The master plate is stored at 4 ° C until the results of the screening procedure become available ... Nitrocellulose Filters 1.
Page 2-112
... filter prior to hybridization . Because more bacteriophage DNA becomes attached to the nitrocellulose filter , the au- toradiographic signals from positive clones are enhanced ... Nitrocellulose Filters Following Situ Amplification 2.
... filter prior to hybridization . Because more bacteriophage DNA becomes attached to the nitrocellulose filter , the au- toradiographic signals from positive clones are enhanced ... Nitrocellulose Filters Following Situ Amplification 2.
Page 7-47
... nitrocellulose filter ( Schleicher and Schuell BA85 or equivalent ) about 1 mm larger than the gel in both dimensions . Use gloves and blunt - ended forceps ( e.g. , Millipore forceps ) to handle the filter . A nitrocellulose filter ...
... nitrocellulose filter ( Schleicher and Schuell BA85 or equivalent ) about 1 mm larger than the gel in both dimensions . Use gloves and blunt - ended forceps ( e.g. , Millipore forceps ) to handle the filter . A nitrocellulose filter ...
Contents
BOOK | 1-2 |
DEVELOPMENT OF PLASMID CLONING VECTORS | 1-7 |
Index | 1-8 |
Copyright | |
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agar agarose gel aliquots amber mutations bacteria bacteriophage M13 bacteriophage particles bacteriophage T4 bacteriophage T4 DNA BamHI Bgill buffer cDNA cells centrifugation at 12,000g cleaved cloning coli DNA polymerase colonies containing cosmid cosmid vector culture digestion DNA fragments DNA ligase DNA molecules DNA polymerase double-stranded DNA EcoRI EDTA efficiency Escherichia coli ethanol ethidium bromide eukaryotic eukaryotic DNA exonuclease extracts foreign DNA gel electrophoresis gene genetic Gp Cp HindIII Hindill host hybridization Incubate infected inserted intergenic region Kpnl lacZ linear lysogenic method methylase methylation microfuge tube minutes at 4°C nin5 nitrocellulose nucleotides origin of replication packaging pellet phagemids plaques plasmid plasmid DNA plate polycloning prepared protein Pvul recA recombinant red gam remove replication restriction enzyme room temperature Sacl Sall single-stranded DNA Smal solution sterile stored strains strand supernatant T4 DNA ligase teriophage Transfer Tris Cl pH vector DNA vitro volume Xbal µg/ml