Molecular Cloning: A Laboratory Manual, Volume 1 |
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Page 1-98
... Nitrocellulose Filters Two methods ( based on the original procedure of Grunstein and Hogness [ 1975 ] ) are described to liberate the DNA from bacterial colonies and to bind it to the nitrocellulose filter in ... Nitrocellulose Filters 1 1.
... Nitrocellulose Filters Two methods ( based on the original procedure of Grunstein and Hogness [ 1975 ] ) are described to liberate the DNA from bacterial colonies and to bind it to the nitrocellulose filter in ... Nitrocellulose Filters 1 1.
Page 2-109
... Nitrocellulose Filters or Nylon Membranes This method is modified from that of Benton and Davis ( 1977 ) . 1. Mix aliquots of a packaging reaction or bacteriophage A stock containing no more than 50,000 bacteriophage particles in a ...
... Nitrocellulose Filters or Nylon Membranes This method is modified from that of Benton and Davis ( 1977 ) . 1. Mix aliquots of a packaging reaction or bacteriophage A stock containing no more than 50,000 bacteriophage particles in a ...
Page 2-112
... Nitrocellulose Filters Following In Situ Amplification Woo et al . ( 1978 ) and Woo ( 1979 ) have described a modification of the Benton and Davis ( 1977 ) screening method that ... Nitrocellulose Filters Following Situ Amplification 2.
... Nitrocellulose Filters Following In Situ Amplification Woo et al . ( 1978 ) and Woo ( 1979 ) have described a modification of the Benton and Davis ( 1977 ) screening method that ... Nitrocellulose Filters Following Situ Amplification 2.
Contents
BOOK | 1-2 |
DEVELOPMENT OF PLASMID CLONING VECTORS | 1-7 |
Index | 1-8 |
Copyright | |
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Common terms and phrases
agar agarose gel aliquots amber mutations bacteria bacteriophage M13 bacteriophage particles bacteriophage T4 bacteriophage T4 DNA BamHI Bgill buffer cDNA cells centrifugation at 12,000g cleaved cloning coli DNA polymerase colonies containing cosmid cosmid vector culture digestion DNA fragments DNA ligase DNA molecules DNA polymerase double-stranded DNA EcoRI EDTA efficiency Escherichia coli ethanol ethidium bromide eukaryotic eukaryotic DNA exonuclease extracts foreign DNA gel electrophoresis gene genetic Gp Cp HindIII Hindill host hybridization Incubate infected inserted intergenic region Kpnl lacZ linear lysogenic method methylase methylation microfuge tube minutes at 4°C nin5 nitrocellulose nucleotides origin of replication packaging pellet phagemids plaques plasmid plasmid DNA plate polycloning prepared protein Pvul recA recombinant red gam remove replication restriction enzyme room temperature Sacl Sall single-stranded DNA Smal solution sterile stored strains strand supernatant T4 DNA ligase teriophage Transfer Tris Cl pH vector DNA vitro volume Xbal µg/ml