Molecular Cloning: A Laboratory Manual, Volume 1 |
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Page 2-98
A Laboratory Manual Joseph Sambrook, E. F. Fritsch, Tom Maniatis. Preparation of Packaging Extracts and Packaging of Bacteriophage λ DNA In Vitro Packaging extracts are available at reasonable cost from commercial sources . These work ...
A Laboratory Manual Joseph Sambrook, E. F. Fritsch, Tom Maniatis. Preparation of Packaging Extracts and Packaging of Bacteriophage λ DNA In Vitro Packaging extracts are available at reasonable cost from commercial sources . These work ...
Page 2-104
... packaging reactions are stored in closed tubes at 4 ° C . Notes i . Each batch of extracts should be tested for packaging efficiency with a standardized preparation of intact bacteriophage › DNA . ii . These extracts exhibit a high ...
... packaging reactions are stored in closed tubes at 4 ° C . Notes i . Each batch of extracts should be tested for packaging efficiency with a standardized preparation of intact bacteriophage › DNA . ii . These extracts exhibit a high ...
Page 3-33
... packaging extracts ( Bates and Swift 1983 ) . Extracts used to package cosmids should be prepared using a buffer that contains spermidine but not putrescine . When putrescine is omitted from the packaging extract and the packaging ...
... packaging extracts ( Bates and Swift 1983 ) . Extracts used to package cosmids should be prepared using a buffer that contains spermidine but not putrescine . When putrescine is omitted from the packaging extract and the packaging ...
Contents
BOOK | 1-2 |
DEVELOPMENT OF PLASMID CLONING VECTORS | 1-7 |
Index | 1-8 |
Copyright | |
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Common terms and phrases
agar agarose gel aliquots amber mutations bacteria bacteriophage M13 bacteriophage particles bacteriophage T4 bacteriophage T4 DNA BamHI Bgill buffer cDNA cells centrifugation at 12,000g cleaved cloning coli DNA polymerase colonies containing cosmid cosmid vector culture digestion DNA fragments DNA ligase DNA molecules DNA polymerase double-stranded DNA EcoRI EDTA efficiency Escherichia coli ethanol ethidium bromide eukaryotic eukaryotic DNA exonuclease extracts foreign DNA gel electrophoresis gene genetic Gp Cp HindIII Hindill host hybridization Incubate infected inserted intergenic region Kpnl lacZ linear lysogenic method methylase methylation microfuge tube minutes at 4°C nin5 nitrocellulose nucleotides origin of replication packaging pellet phagemids plaques plasmid plasmid DNA plate polycloning prepared protein Pvul recA recombinant red gam remove replication restriction enzyme room temperature Sacl Sall single-stranded DNA Smal solution sterile stored strains strand supernatant T4 DNA ligase teriophage Transfer Tris Cl pH vector DNA vitro volume Xbal µg/ml