Molecular Cloning: A Laboratory Manual, Volume 1 |
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Results 1-3 of 61
Page 1-25
... pellet as dry as possible . The supernatant can be conveniently removed with a disposable pipette tip attached to a vacuum line ( see Figure 1.3 ) . Use a gentle vacuum and touch the tip to the surface of the liquid . Keep the tip as ...
... pellet as dry as possible . The supernatant can be conveniently removed with a disposable pipette tip attached to a vacuum line ( see Figure 1.3 ) . Use a gentle vacuum and touch the tip to the surface of the liquid . Keep the tip as ...
Page 1-29
... pellet ( obtained in step 3 , page 1.25 ) in 350 μl of STET . STET 0.1 M NaCl 10 mM Tris Cl ( pH 8.0 ) 1 mM EDTA ( pH 8.0 ) 5 % Triton X - 100 2. Add 25 μl of a freshly prepared solution of lysozyme ( 10 mg / ml in 10 mм Tris Cl [ pH ...
... pellet ( obtained in step 3 , page 1.25 ) in 350 μl of STET . STET 0.1 M NaCl 10 mM Tris Cl ( pH 8.0 ) 1 mM EDTA ( pH 8.0 ) 5 % Triton X - 100 2. Add 25 μl of a freshly prepared solution of lysozyme ( 10 mg / ml in 10 mм Tris Cl [ pH ...
Page 7-81
... pellet of nucleic acids that has been precipitated with ethanol . This problem is exacerbated if the pellet is dried in a desiccator . Sometimes the pellet can be redissolved by a combination of vigorous pipetting and heating to 60 ° C ...
... pellet of nucleic acids that has been precipitated with ethanol . This problem is exacerbated if the pellet is dried in a desiccator . Sometimes the pellet can be redissolved by a combination of vigorous pipetting and heating to 60 ° C ...
Contents
BOOK | 1-2 |
DEVELOPMENT OF PLASMID CLONING VECTORS | 1-7 |
Index | 1-8 |
Copyright | |
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Common terms and phrases
agar agarose gel aliquots amber mutations bacteria bacteriophage M13 bacteriophage particles bacteriophage T4 bacteriophage T4 DNA BamHI Bgill buffer cDNA cells centrifugation at 12,000g cleaved cloning coli DNA polymerase colonies containing cosmid cosmid vector culture digestion DNA fragments DNA ligase DNA molecules DNA polymerase double-stranded DNA EcoRI EDTA efficiency Escherichia coli ethanol ethidium bromide eukaryotic eukaryotic DNA exonuclease extracts foreign DNA gel electrophoresis gene genetic Gp Cp HindIII Hindill host hybridization Incubate infected inserted intergenic region Kpnl lacZ linear lysogenic method methylase methylation microfuge tube minutes at 4°C nin5 nitrocellulose nucleotides origin of replication packaging pellet phagemids plaques plasmid plasmid DNA plate polycloning prepared protein Pvul recA recombinant red gam remove replication restriction enzyme room temperature Sacl Sall single-stranded DNA Smal solution sterile stored strains strand supernatant T4 DNA ligase teriophage Transfer Tris Cl pH vector DNA vitro volume Xbal µg/ml